넙치 치어 Paralichthys olivaceus의 혈액학적 성상에 미치는 나프탈렌의 급성독성영향 The Acute Toxicity of Naphthalene on Hematologic Properties in Juvenile Flounder Paralichthys olivaceus
pp.191-196
넙치 치어에 대한 나프탈렌의 급성독성을 조사하기 위하여 대조구(0μg/L), 1000, 1800, 3200, 5600, 10000μg/L의 6개의 나프탈렌 농도구를 설정하여 24시간 동안 노출실험을 실시하고 혈액학적 성상을 분석하였다. 넙치 치어에 대한 나프탈렌의 24h-LC50은 3600μg/L를 나타냈다. 넙치 치어의 핼액학적 반응에서 헤마토크리트값은 5600, 10000μg/L의 농도구에서 대조구와 비교하여 유의하게 증가하였고, 글루코스는 10000μg/L의 농도 구에서 유의하게 증가 하였다(P〈0.05). 삼투질 농도는 3200, 5600, 10000μg/L의 농도구에서 유의하게 증가하였다. 반면, 이온분석 결과 [Na+], [K+]은 5600 및 10000μg/L 나프탈렌 농도구에서 유의하게 증가하였으나 [Cl-]는 큰 차이를 보이지 않았다.
Naphthalene was composed of a substantial fraction of polycyclic aromatic ydrocarbons(PAHs) in crude oil and causes acute toxicity. In this study, we examined the toxicity of different kinds concentrations 0, 1000, 1800, 3200, 5600, 10000μg/L of naphthalene to juvenile flounder, Paralichthys olivaceus for 24h to determine 24-median lethal concentration(LC50) and acute effect on the hematological properties. 24h-LC50 value of this species was 3600μg/L. Hematocrit value significantly increased at 5600 and 10000μg/L naphthalene exposed group by 24h compared to control fish. Plasma. Glucose was significantly higher in the 10000μg/L (P〈0.05). Plasma osmolality was significantly higher in the 3200, 5600 and 10000μg/L. Plasma [Na+] and [K+] significantly increased in the 5600 and 10000μg/L, however [Cl-] was not affected by acute naphthalene exposure. The results of this study suggest the acute exposure to naphthalene affects both ionoregulation and osmoregulation in juvenile flounder.