This research sought to analyze the characteristics of breast movement at the treadmill activity levels. It also examined the effect of wearing a sports bra in reducing breast displacement. The subjects for the data collection were females in their 20s (n=2) with C-cup size breast. The experimental conditions were three different moving speeds (4 km/h, jogging: 7 km/h, and sprinting: 10 km/h) and two types of sports bras. Three dimensional breast displacement was measured. The displacement of the right nipple point was measured with a 3D motion analyzer. The results show that the breasts were greatly displaced from the walking speed (4 km/h) when subjects did not wear any bra. Whereas their breast displacement distance decreased remarkably when they wore sports bras. The nipple point moved 42~44 mm in the vertical direction at walking speed with naked condition. But it was reduced by 80% after wearing sports bras. When subjects running (7 km/h, 10 km/h) without any bra, the nipple point moved 122~141 mm. However it was reduced by 60~70% when they wore sports bras. The apartment time (time delay) between at the highest point of the upper body and the nipple was 0.25 seconds at the running speeds (7 km/h, 10 km/h) without wearing any bra. After wearing sports bras, the time delay was cut to 0.06~0.12 seconds. These results implies that without wearing any bra the skin surrounding the breasts might be seriously pulled at running activity. The functional sports bra suppress breast movement. It might prevent the sagging of breasts by preventing the damage of the Cooper's ligaments.