KOREASCHOLAR

As pulp calcification occurs at least fifty percent of total teeth, the focal calcification in pulp chamber usually appears in all age groups. However, the pulp calcification is one of the important pathologic changes affecting the pulp vitality. In order to elucidate the mechanism of pulp calcification during the retrogressive degeneration of pulp tissue we performed an immunohistochemical study for proteases (MMP-3, MMP-10, and cathepsin-G), antiproteases (TIMP-1, α1- AT) and proteins involving tissue protection (TGase-2 and HSP-70). In the normal pulp tissue MMP-3 and MMP-10 were weakly expressed, but cathepsin-G and TIMP-1 were rarely expressed. Around the calcifying tissue of MMP-3, MMP- 10, and α1-AT were predominant, but TIMP-1 and cathepsin-G were sparsely expressed. On the other hands, TGase-2 and HSP-70 were condensed in the proximal fibrous tissue. These data suggest that the pulp calcification is related to retrogressive pulp degeneration, which could be resulted in the incomplete digestion of the degenerated stromal tissue by different proteases. We presume that the aberrant protease digestion of chronic pulpal pathosis, i.e., sclerotic fibrosis, chronic pulp degeneration, etc., may enhance the dystrophic calcification in dental pulp.

I. 서 론
 II. 실험재료 및 연구 방법
  1. 실험대상
  2. 조직 채취 및 조직절편 제작
  3. 면역조직화학적 염색
 III. 연구 결과
 IV. 총괄 및 고찰
 참고문헌

• 김진우(강릉대학교 치과대학 보존학교실) | Jin Woo Kim
• 김현희(강릉대학교 치과대학 보존학교실) | Hyun Hee Kim
• 이상신(강릉대학교 치과대학 구강병리학 교실) | Sang Shin Lee
• 이영준(강릉대학교 치과대학 구강병리학 교실) | Young Joon Lee
• 이장하(강릉대학교 치과대학 구강병리학 교실) | Jang Ha Lee
• 김연숙(강릉대학교 치과대학 구강병리학 교실) | Yeon Sook Kim
• 이석근(강릉대학교 치과대학 구강병리학 교실) | Suk Keun Lee Corresponding Author
• 문혁수(서울대학교 치과대학 예방치학 교실) | Hyock Soo Moon
• 이종헌(단국대학교 치과대학 구강병리학 교실) | Chong Heon Lee