KOREASCHOLAR

Established SGT cell line from human submandibular gland adenocarcinoma was used to study the TGase expression on a cellular level in vitro. Transglutaminase 2(TGase 2) is assoacitated with apoptosis, GTP binding protein, and cell marix interaction. The role of TGase 2 in salivary gland tumors is not clear yet. The pupose of this study were to examine the TGase expression of SGT cell line compared to other tumor cell lines, and to apply these results to the pathogenesis of salivary gland tumor. TGase enzyme assay of SGT, SCC-15, HN 4 and HeLa tumor cell line was 3 times repeated, and calculated. Immunoslot blot for semiquantitative protein analysis was done. The obtained results were as follows.
1. SGT cell line showed the highest TGase 2 enzyme activity(about 6-16 folds) irrespective of pre or postconfluency.
2. HN 4 cell line showed the highest TGase 1 enzyme activity(about 2-3 folds) irrespective of pre or postconfluency.
3. Under postconfluency TGase 1 induction was not induced, but slightly increased in all tumor cell lines.
4. TGase enzyme activity in all tumor cell lines was accompanied with TGase protein formation.
From the aboving results, the higher TGase 2 expression of SGT cell line suggested that they would come from submandibular ductal cells and have a important role in the pathogensis of salivary gland tumors.

I. INTRODUCTION
 II. MATERIALS and METHODS
  1. Culture conditions of Tumor cell line
  2. Morphologic observation
  3. Transglutaminase activity assay
  4. Immunoslot Blot
 III. RESULTS
  1. Morphologic features
  2. Tranglutaminase activity assay
  3. Immunoslot blot
 IV. DISCUSSIONS
 REFERENCES

• 이동식(Dental College, Dankook University) | Dong Sik Lee
• 유종우(Dental College, Dankook University) | Woo Jong You
• 신응섭(Dental College, Dankook University) | Eung Sup Shin
• 이병헌(Dental College, Dankook University) | Byung Heon Lee
• 이종헌(Dental College, Dankook University) | Chong Heon Lee correspondence
• 홍성철(Department of Oral Pathology and Oral Physiology) | Seong Cheol Hong