악구강계 편평세포암종 세포주에서 HPV mRNA 인지 Detection of HPV mRNA in HNSCC cell lines
대한구강악안면병리학회지
제27권 제2호
(2003.06)
pp.139-143
pp.139-143
대한구강악안면병리학회
(Korean Academy Of Oral And Maxillofacial Pathology)
초록
E6 and E7 as two major oncoproteins of HPV can act together to produce several tumors, providing an evidence for the role HPV in oral squamous cell tumorogenesis. It is worthy to detect E6/E7 mRNA expression of HPV in oral carcinoma. The purpose of this study were to detect HPV mRNA in HNSCC cell lines, and to use these results to confirm oral squamous cell carcinoma. Semi-quantitative RT-PCR method for E7 mRNA expression in HNSCC cell lines should play an important role in detecting the early stage of oral squamous cell carcinoma.
목차
I. INTRODUCTION
II. MATERIALS and METHODS
A. Culture Conditions
B. Total RNA extraction from NHOK, IHOK,Caski cell line and HNSCC cell lines for RT-PCR
C. RT-PCR reaction for cDNA
III. RESULTS
IV. DISCUSSION
REFERENCES
II. MATERIALS and METHODS
A. Culture Conditions
B. Total RNA extraction from NHOK, IHOK,Caski cell line and HNSCC cell lines for RT-PCR
C. RT-PCR reaction for cDNA
III. RESULTS
IV. DISCUSSION
REFERENCES
저자