KOREASCHOLAR

Genomic imprinting is defined as parent-of-origin expression of specific genes and may play an important role in embryonal development of mammals. Loss of imprinting(LOI), biallelic expression of the imprinted genes, have been observed in a variety of human tumors and syndromes. H19, a paternally imprinted gene, is transcribed as an untranslated RNA that serves as a riboregulator. LOI of H19 is observed in a variety of human malignancies. In this study, LOI of H19 was examined in head and neck squamous cell carcinomas(HNSCCs). Four(28.6%) of the 14 HNSCCs and 8(28.6%) of the 28 inflammatory oral lesions were informative for imprinting analysis of H19. H19 was imprinted in all inflammatory oral lesions, however, 2(50%) of the 4 informative HNSCCs manifested LOI. These data suggest that LOI of the H19 may play a role in the oncogenesis of HNSCC.

I. 서 론
 II. 재료 및 방법
  1. Genomic DNA 추출
  2. Genomic polymorphism의 확인
  3. RNA 추출
  4. cDNA 합성
  5. H19 LOI 분석
 III. 연구결과
  1. Genomic polymorphism의 확인
  2. H19 loss of imprinting(LOI) 분석
 IV. 고 찰
 V . 결 론
 VI. 참 고 문 헌

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