불멸화 구강각화세포에서 다양한 사이토 카인의 발현 Various Cytokine Expression of Immortalized Human Oral Keratinocyte
대한구강악안면병리학회지
제30권 제3호
(2006.06)
pp.151-155
pp.151-155
대한구강악안면병리학회
(Korean Academy Of Oral And Maxillofacial Pathology)
초록
Pro-inflammatory cytokines are important mediators of cutaneous cellular activities during many oral mucosal diseases. IHOK culture model transfected by E6/E7 genes provide further evidence for the role of HPV in tumorogenesis. It is interesting to investigate cytokine expression of immortalized human oral keratinocyte(IHOK). The purpose of this study were to analysis cytokine mRNA expression levels of NHOK and IHOK by RT-PCR. IHOK showed about 5 fold increases of IL-6 compared with NHOK, while TNF-α was the lowest. It suggested that immortalization of NHOK with E6/E7 could result in elevated expression of IL-6, and IHOK be in the intermediate stage of oral carcinogenesis.
목차
I. INTRODUCTION
II. MATERIALS and METHODS
1. Cell culture condition
2. Total RNA extraction from NHOK and IHOKfor RT-PCR
3. RT-PCR reaction for cDNA
III. RESULTS
IV. DISCUSSION
REFERENCES
II. MATERIALS and METHODS
1. Cell culture condition
2. Total RNA extraction from NHOK and IHOKfor RT-PCR
3. RT-PCR reaction for cDNA
III. RESULTS
IV. DISCUSSION
REFERENCES
저자