KOREASCHOLAR

Nuclear factor I-C (NFI-C) null mice demonstrated aberrant odontoblast differentiation, abnormal dentin formation, and thus molar lacking roots. However, the mechanism by which the disruption of NFI-C gene affect the expression of other genes in dental pulp cells remains unknown. In this study, in order to understand this mechanism, the gene expression of pulp cells in NFI-C deficient mice were compared to those of wild-type mice by cDNA microarray analysis. According to the cDNA microarray profile comparison, the disruption of NFI-C gene increased the expression of TGF-β and TGF-β receptor, whereas it decreased the expression of Smad proteins. Interestingly, most of the FGF-related genes were down-regulated in pulp cells by NFI-C gene disruption. Among the cell cycle-related genes, the expression of p16 and p18 were increased by NFI-C disruption, but the expression of cy clin E1 and cy clin D1 were decreased by NFI-C disruption. These results indicate that the disturbance of NFI-C gene suppressed the proliferation of pulp cells and up-regulated the expression of TGF-β and its downstream signaling molecules during root formation, contributing to the formation of short root containing abnormal dentin.

I. 서론
 II. 재료 및 방법
  1. 정상 생쥐와 NFI-C 결손 생쥐의 치수세포배양
  2. RNA 분리 및 순도 측정
  3. cDNA microarray
  4. RT-PCR 분석
  5. Western blot 분석
 III. 실험결과
  1. RNA 순도와 microarray의 scatter plot 분석
  2. TGF-β/BMP 신호전달경로에 연관된 유전자군의 분석
  3. Map-Kinase 신호전달경로에 연관된 유전자군의 분석
  4. Cell cycle과 연관된 유전자군의 분석
 IV. 총괄 및 고안
 V. 참고 문헌

• 이동설(서울대학교 치과대학 발생생물·구강조직학교실 및 치학연구소) | Dong Seol Lee
• 김성일(조선대학교 치과대학 구강해부학교실) | Sung Il Kim
• 한평호(조선대학교 치과대학 구강해부학교실) | Pyung Ho Han
• 김흥중(조선대학교 치과대학 구강해부학교실) | Heung Joong Kim
• 김현만(서울대학교 치과대학 발생생물·구강조직학교실 및 치학연구소) | Hyun Man Kim
• 고재승(서울대학교 치과대학 발생생물·구강조직학교실 및 치학연구소) | Jea Seung Ko
• 손호현(서울대학교 치과대학 치과보존학교실 및 치학연구소) | Ho Hyun Son
• 박주철(서울대학교 치과대학 발생생물·구강조직학교실 및 치학연구소) | Joo Cheol Park correspondence