탈회동결건조골과 수종의 생물학적 물질들을 티타늄 매식체와 함께 이식 후 골형성 Bone Formation after Grafting of Various Mixture of Demineralized Freeze Dried Bone and Several Biologic Factors with Titanium Implant
pp.241-252
Demineralized Freeze Dried Bone(DFDB) graft material have been used for reconstruction of large bony defects or augmentation of thin alveolar ridge during implantation of titanium fixtures. But at present osteogenic effect of DFDB do not overcome the capacity of autogenic bone graft. Thus many investigators had applicated various bioactive substance to DFDB to enhance the ability of osteogenesis of DFDB. In this study, mixture of grafting material was made from fibrin glue(F) and DFDB(D)(group 1: F+D), fibrin glue, DFDB and rhBMP-2(B) (group 2: F+D+B), fibrin glue, DFDB, polylactic- co-glycolic acid(PLGA)(P) and rhBMP-2(goup 3: F+D+B+P), fibrin glue, DFDB, PLGA, rhBMP-2 and autogenic osteoblasts( O)(group 4: F+D+B+P+O), and fibrin glue, DFDB, autogenic osteoblasts (group 5: F+D+O). During first surgical procedure, extraction of molar teeth was performed at male Biggle dog's mandible, and collected bone marrow tissue from tibia at same Biggle dog. Collected bone marrow tissue was cultured and differentiated into osteoblasts in vitro, and stored in nitrogen bottle. After four months, titanium fixture was implanted with prepared graft material to Biggle dog's mandible. After four and eight weeks respectively, experimental dog was sacrificed. Obtained tissues were prepared for examination by using resin embedded ground section method. Prepared sections were evaluated with transmitted and polarized microscope, and areas of osteoid and cacified bone were calculated with IPTK 5.0( image processing tool kit version 5.0). Resultant data was statistically analyzed by SPSS 13.0 software. Results of this study showed that autogenic osteoblats had more enhancing capacity of bone formation than rhBMP-2, but PLGA inhibited bone forming potential of bony tissue.
II. 재료 및 방법
1. 실험군의 편성
2. 실험동물 및 외과적 처치
3. 골수세포의 채취
4. 조골세포의 분리 및 배양
5. 연마표본의 제작
6. fibrin glue, DFDB 그리고 rhBMP-2 혼합물(실험 2군) 및 fibrin glue, DFDB, rhBMP-2그리고 PLGA혼합물(실험 3군)의 준비
7. 표본의 관찰
8. 통계분석
III. 결과
1) 실험 4주군과 8주군간의 비교
2) 실험 4주군간 비교
IV. 총괄 및 고찰
V. 결론
VI. 참고문헌