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비쑥의 휘발성물질에 의한 KB 세포의 세포사멸 유도 Induction of Apoptosis in Human Oral Epidermoid Carcinoma Cell by The Essential Oil of Artemisia Scoparia WALDST. et KITAMURA

차정단, 이생금, 이명섭, 차인호
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  • URLhttp://db.koreascholar.com/Article/Detail/292916
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대한구강악안면병리학회지
제33권 제2호 (2009.04)
pp.75-82
대한구강악안면병리학회 (Korean Academy Of Oral And Maxillofacial Pathology)
초록

Artemisia scoparia (A. scoparia), perennial herb is indigenous to Korea and has been traditionally used in liver damage. We investigated the effect of the essential oil obtained from A. scoparia on apoptosis of KB cells. Cytotoxicity and cellular DNA content were analyzed by MTT assay and flow cytometry, agarose gel electrophoresis, and Hoechst 33258 staining. The caspase-3 and poly (ADP-ribose) polymerase (PARP) proteins were estimated by Western blotting method. We found that the essential oil induced the apoptosis of the KB cells by concentrations of 0.4 to 0.2 mg/ml which was verified by DNA fragmentation, apoptotic bodies, and the sub-G0/G1 ratio. The essential oil also transient caspase-9 and caspase-3 activity and cleavage of PARP in KB cells for 24 h. The essential oil-induced apoptotic cell death was accompanied by up-regulation of Bax and down-regulation of Bcl-2. In conclusion, we demonstrated that the essential oil of A. scoparia induces apoptosis in KB cells

키워드
Artemisia scopariaEssential oilApoptosisCell cycleCaspase-3PARP
목차
I. INTRODUCTION
 II. MATERIALS AND METHODS
  1. Plant material
  2. Isolation of the essential oil
  3. Cell culture
  4. Cell viability
  5. Cell cycle analysis
  6. Nuclear morphology analysis
  7. DNA extraction and DNA gel electrophoresis
  8. Preparation of cytosolic and mitochondrialextract
  9. Western blotting
  10. Statistical analysis
 III. RESULTS
  1. Essential oil of A. scoparia induces cytotoxicityin a dose-dependent manner
  2. Effect of the essential oil on cell cyclephase distribution in KB cells
  3. Effect of the essential oil on determinationof morphological changes in KB cells
  4. Effect of the essential oil on DNA fragmentationin KB cells
  5. Mitochondrial stress and activation ofcaspase-9 and -3 and cleavage of PARP inthe essential oil-mediated apoptosis of KB
 IV. DISCUSSION
 V. REFERENCES
저자
  • 차정단(Department of Oral Cancer Research Institute) | Jeong-Dan Cha1
  • 이생금(Department of Oral Cancer Research Institute) | ShengJin, Li
  • 이명섭(Department of Oral & Maxillofacial Surgery) | Myeong-Seop Lee
  • 차인호(Department of Oral Cancer Research Institute, Department of Oral & Maxillofacial Surgery) | In-Ho Cha correspondence