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CDK4와 hTERT를 도입한 HPV16 E6/E7 불멸화 세포의 확립 Establishment of CDK4/hTERT-Transfected HPV16 E6/E7 Immortalized Cell Lines

박영진, 홍두표, 김은철, 오봉경, 김진
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대한구강악안면병리학회지
제34권 제1호 (2010.02)
pp.27-36
대한구강악안면병리학회 (Korean Academy Of Oral And Maxillofacial Pathology)
초록

Human papillomavirus (HPV) has been classified as one of the causing factors of head and neck squamous cell carcinoma (HNSCC). However, little is known about HPV-related carcinogenesis in HNSCC. The purpose o f this s tudy i s to characterize immortalized human oral keratinocyte (IHOK) transfected by HPV16 E6/E7, IHOK/hcdk4 (IHOK transfected by pLXRN-hcdk4) and IHOK/hcdk4/hTERT (IHOK transfected by pLPC-hTERT-hcdk4) to reconstitute HNSCC in vitro. Conclusively, we established a new immortalized cell lines, IHOK/hcdk4 and IHOK/hcdk4/hTERT, to understand multistep carcinogenic process of oncogenic HPV16 E6/E7 in HNSCC.

키워드
hcdk4HPV16 E6/E7hTERTImmortalization
목차
I. 서 론
 II. 재료 및 방법
  1. 세포 배양
  2. PD(population doublings) 측정
  3. PCR과 RT-PCR
  4. TRAP(Telomeric repeat amplificationprotocol) assay
  5. 단백질 발현(Western blotting)
 III. 결 과
  1. 불멸화 세포주의 형태학적인 특징과 성장 속도
  2. PCR을 이용한 HPV16 E6와 E7의 존재와 변이여부
  3. p53, hcdk4, Rb 그리고 pRb의 단백질 발현
  4. Telomerase activity와 hTERT의 발현
 IV. 고 찰
 V. 참고문헌
저자
  • 박영진(연세대학교 치과대학 구강병리학교실, 구강종양연구소) | Young Jin Park
  • 홍두표(연세대학교 치과대학 구강병리학교실, 구강종양연구소) | Doo Pyo Hong
  • 김은철(BK21 치의과학 사업단,원광대학교 치과대학 구강병리학교실) | Eun Cheol Kim
  • 오봉경(연세 암 센터) | Bong Kyeong Oh
  • 김진(연세대학교 치과대학 구강병리학교실, 구강종양연구소) | Jin Kim correspondence