KOREASCHOLAR

EGCG has inhibitory effect on a variety of cancers by inducing apoptosis and cell cycle arrest or inhibiting angiogenesis and metastasis. EGCG has been found to induce apoptosis in salivary gland carcinoma cells. But the potential anti-invasive effect of EGCG in salivary gland cancer has not been studied yet. The aim of this study is to evaluate the effect of EGCG on salivary gland adenocarcinoma SGT cell adhesion and migration to Type I collagen treatment. Western blot, adhesion and migration assay were performed to evaluate the impacts of EGCG on the expression of MMP-2/-9 and its upstream signaling molecules after treatment of type I collagen. SGT cell adhesion to type I collagen is significantly suppressed by EGCG. EGCG decreased expression of β1 integrin, phosphorylation of FAK, MMP-2/-9 compared with type I collagen treatment. In addition, EGCG inhibited the migration of SGT cells treated with type I collagen. These results suggest that EGCG could effectively inhibit the invasion and migration of human SGT cells by downregulating the expression of β1 integrin and MMP-2/-9 and phosphorylation of FAK, Akt, and Erk. Adhesion and migration to type I collagen of SGT cells can be influenced through EGCG.

Ⅰ. 서론
 Ⅱ. 연구재료 및 방법
  1. 세포 배양 및 MTT 분석
  2. 세포부착 실험(Cell adhesion assay)
  3. 세포이동 실험(Cell migration assay)
  4. Western blot analysis
  5. Statistical analysis
 Ⅲ. 결과
  1. SGT 세포의 type I collagen 부착에 대한EGCG의 영향
  2. Type I collagen 처리 후 EGCG에 의한단백질 발현 변화
  3 . Type I c ollagen 처리 후 SGT 세포 이동에서EGCG의 효과
 Ⅳ. 고찰
 Ⅴ. 참고문헌

• 윤효은(조선대학교 치과대학 병리학교실) | Hyo Eun Yoon
• 윤정훈(조선대학교 치과대학 병리학교실) | Jung Hoon Yoon correspondence
• 안상건(조선대학교 치과대학 병리학교실) | Sang Gun Ahn correspondence