KOREASCHOLAR

Human β- defensin-2 (hBD-2) is an antimicrobial peptide which is produced by epithelial cells after stimulation with microorganisms or inflammatory mediators. However, little is known as to whether the LPS and nicotine induces the expression of hBD-2 in periodontal l igament (PDL) cells. T he p urpose o f this s tudy was t o investigate t he r oles o f MAPKs pathway o f nicotine a nd L PS-induced hBD-2 expression in PDL cells. The maximal expression of hBD-2 was observed in nicotine 5 mM and LPS 1 μg/ml treated PDL cells. Nicotine and LPS induced the phosphorylation of p38, c-Jun N-terminal kinase 1 and 2 (JNK1/2), and extracellular signal-regulated kinases 1 and 2 (ERK1/2) MAPK. ERK1/2 inhibitor SB203580, p38 inhibitor PD98059, and JNK inhibitor SP600125, blocked the effects of nicotine and LPS on hBD-2 upregulation in PDL cells. These results collectively suggest that hBD-2 is up-regulated in nicotine and LPS-treated PDL cells through activation of p38, ERK and JNK MAPKs pathway. Our data regarding the up-regulation of hBD-2 may help us to understand the antimicrobial mechanism in periodontal disease

Ⅰ. 서론
 Ⅱ. 재료 및 방법
  1. 세포배양
  2. Western 분석
  3. EIA에 의한 PGE2 측정
  4. 통계처리
 Ⅲ. 결과
  1. 니코틴과 LPS에 의한HBD-2와 COX-2 단백질의 발현
  2. 니코틴과 LPS에 의한 MAPK 인산화에 대한 영향
  3. 니코틴과 LPS에 의한 HBD-2, COX-2단백 유도 및PGE 생산에서 MAPK 억제자의 효과
 Ⅳ. 고찰
 Ⅴ. 결론
 Ⅵ. 참고문헌

• 이상임(경희대학교 치의학전문대학원 악안면재생학교실, 원광대학교 치과대학 구강악안면병리학교실) | Sang Im Lee
• 이화정(경희대학교 치의학전문대학원 악안면재생학교실) | Hwa Jeong Lee
• 배원정(경희대학교 치의학전문대학원 악안면재생학교실) | Won Jung Bae
• 강순일(경희대학교 치의학전문대학원 악안면재생학교실) | Sun IL Kang
• 이소윤(경희대학교 치의학전문대학원 악안면재생학교실, 원광대학교 치과대학 구강악안면병리학교실) | So Youn Lee
• 이영만(경희대학교 치의학전문대학원 악안면재생학교실) | Young Man Lee
• 신경섭(원광대학교 치과대학 구강악안면병리학교실) | Kyoung Seob Shin
• 박재국(원광대학교 치과대학 치과교정학교실) | Jae Kook Park
• 류원국(원광대학교 치과대학 치과교정학교실) | Won Kook Ryu
• 채종문(원광대학교 치과대학 치과교정학교실) | Jong Moon Chae
• 김은철(경희대학교 치의학전문대학원 악안면재생학교실) | Eun Cheol Kim correspondence