Pituitary tumor transforming gene 1(PTTG1)가 구강 편평상피세포암 이동에 미치는 영향 Effect of Pituitary Tumor Transforming Gene 1 in Oral Squamous Cell Carcinoma for Migrat
pp.187-194
Human pituitary tumor transforming gene 1 (PTTG1), which is a newly identified proto-oncogene, is highly expressed in normal pituitary tissues containing proliferating cells and in several cancers. Also, PTTG1 has been known as a securin to involve in the regulation of c ell-cycle and in t he p rogression o f tumor. B u t the effect o f PTTG1 in o ral squamous cell carcinoma (oral SCC) h as not b een studied yet. The objective of this study is to analyze the expression of PTTG1 in oral SCC cell lines (YD-10B and YD-15) and to evaluate the effect of PTTG1 on oral SCC cell lines for the migration effect by PTTG1 siRNA treatment. Western blot, migration assay, and zymography were performed to evaluate the effects of PTTG1 on the expression of MMP-2/-9 and migration activity after PTTG1 siRNA treatment. PTTG1 was expressed in oral SCC cells lines, otherwise, significantly decreased after PTTG1 siRNA treatment. There is no difference in expression of MMP-2 regardless of PTTG1 siRNA treatment. However, the enzyme activity of MMP-9 was significantly decreased. In addition, the migration activities of oral SCC cells were significantly decreased after PTTG1 siRNA treatment (p<0.001). These results suggested that the down-regulated PTTG1 could inhibit the migration of human oral SCC cells through the low MMP-9 expressions. Therefore, these findings provide a useful guideline for the migration mechanism of oral SCC depend on PTTG1 expression.
Ⅱ. 재료 및 방법
1. 세포 배양 및 MTT 분석
2. PTTG1 siRNA 실험
3. RT-PCR analysis
4. W estern b lot analysis
5. Migration assay
6. Gelatin zymography analysis
Ⅲ. 결과
1. YD-10B와 YD-15 세포의 증식 및 특징분석
2. PTTG1 siRNA 처리에 따른 YD-10B와 YD-15 세포주에서의 PTTG1 발현 변화
3. PTTG1 발현 감소에 따른 YD-10B 세포와 YD-15세포의 이동 억제 효과
Ⅳ. 고찰
Ⅴ. 참고문헌