KOREASCHOLAR

Urokinase-type plasminogen activator(uPA) bound to urokinase plasminogen activator receptor(uPAR) expression is strongly correlated with the metastatic potential of various tumors by enhancing ECM degradation through plasminogen and matrix metallopreotease activation. But expression of uPA/uPAR in human malignant salivary gland tumors has been rarely reported. The purpose of this study were to investigate mRNA expression and cytologic concentration of uPAR in SGT cell line compared to various cancer cell lines by RT-PCR and ELISA method, and to study migration and adhesion assay. These results would be to apply the pathogenesis and prognosis of malignant salivary gland tumors. All the cell lines(SGT, HN 4, SCC 25, and HeLA) were cultured under DMEM with 10% FBS at 37℃ in a 5% CO2 incubator. We studied a possible association between mRNA expression and cytosolic concentrations of uPAR in SGT cell line compared to various cancer cell lines using RT-PCR and an enzyme-linked immunoassay( ELISA) method. And also cell adhesion and migration assay were done in all the cell lines. In migration assay SGT cell line was about 2.5-4 folds higher than another cell lines. In adhesion assay SGT cell line was about 1.1-2 folds higher than another cell lines. uPAR cytosolic concentrations of SGT cell line was about 3.4-10 folds by ELISA, while mRNA expression was about 2.5-5 folds by RT-PCR. Oral Scc cell lines showed the lowest value. uPAR protein and mRNA expression were correlated with migration and adhesion assay. From the aboving results, high cytosolic concentrations and mRNA expression of uPAR were correlated with migration and adhesion assay. It suggested that this might be a specific marker for malignant potential of SGT cell line and would be contributed to pathogenesis and prognosis of human salivary gland adenocarcinoma

Ⅰ. INTRODUCTION
 Ⅱ. MATERIALS AND METHODS
  1. Culture Condition
  2. Cell Migration Assay
  3. Cell Adhesion Assay
  4. Detection of uPAR mRNA by RT-PCR
  5. ELISAs Analysis
 Ⅲ. RESULTS
 Ⅳ. DISCUSSION
 Ⅴ. REFERENCES

• 최현식(Department of Oral Pathology, Dental College, The Oral Aging Research Center, Dankook University) | Hyun Sik Choi
• 박경주(Oral Histology, Dental College, The Oral Aging Research Center, Dankook University) | Gyeong Ju Park
• 천윤권(Department of Oral Pathology, Dental College, The Oral Aging Research Center, Dankook University) | Yun Gueon Cheon
• 이종헌(Department of Oral Pathology, Dental College, The Oral Aging Research Center, Dankook University) | Chong Heon correspondence