KOREASCHOLAR

Topoisomerases are essential enzymes involved in all processes of DNA metabolism, and their inhibitors have been identified as potential anti-cancer agents. The present study examined the effect of linoelaidic acid (C18 polyunsaturated fatty) compounds derived from Gardenia jasminoids Ellis extract on the activity of eukaryotic topoisomerases inhibition. The present study identified linoelaidic acid compounds using open column fraction, HPLC, NMR and LC/MS which have effects on cell death in oral cancer cell line, FaDu, but not in immortalized normal cell line, HaCaT. Subsequent studies revealed linoelaidic acid-induced autophagy through LC3 activation. Finally, its inhibition of topoisomerase I and selectively induction of oral cancer cell death possibly implies that linoelaidic acid can be a role as potenial agents in the prevention and therapy of oral cancer.

Ⅰ. Introduction
 Ⅱ. Material and Method
  1. Plant material extraction
  2. Topoisomerase I inhibition assay
  3. Open column chromatography
  4. High-performance liquid chromatography analysisand preparation
  5. Structural elucidation
  6. MTT assay
  7. Histomorphologic evaluation by Diff-QuickⓇstaining
  8. Caspase-8, 9 and caspase-3 activity assay
  9. Immunoblotting
 Ⅲ. Results
  1. Topoisomerase I inhibitory activity of Gardeniajasminoides Ellis by extracts using varioussolvents
  2. Dichloromethane extract of Gardenia jasminoidsEllis separation using by open column fraction
  3. High performance liquid chromatograpy in 90 %and 100 % methanol open column fraction ofGardenia jasminoids Ellis
  4. Structural analysis of topoisomerase I inhibitorin Gardenia jasminoids extract
  5. Topoisomerase I inhibition acitivity of linoelaidicacid
  6. Effects on cell viability of linoelaidic acid inHaCaT and FaDu cells.
  7. Caspase-8, 9 and -3 activity assay of linoelaidicacid
  8. LC3 protein expression by linoelaidic acidstreatment
 Ⅳ. Discussion
 Ⅴ. References

• 고영종(School of Dentistry, Chonnam National University Department of Oral Pathology) | Young Jong Ko
• 최창우(School of Dentistry, Chonnam National University Department of Oral Pathology) | Chang Woo Choi
• 임원봉(School of Dentistry, Chonnam National University Department of Oral Pathology) | Won Bong Lim
• 김지선(School of Dentistry, Chonnam National University Department of Oral Pathology) | Ji Sun Kim
• 김상우(School of Dentistry, Chonnam National University Department of Oral Pathology) | Sang Woo Kim
• Sandeep Karna(School of Dentistry, Chonnam National University Department of Oral Pathology)
• Zeng Hui(School of Dentistry, Chonnam National University Department of Oral Pathology)
• 전상미(School of Dentistry, Chonnam National University Department of Oral Pathology) | Sang Mi Jeon
• 조민성(School of Dentistry, Chonnam National University Department of Oral & Maxillofacial Surgery) | Min Sung Cho
• 최홍란(School of Dentistry, Chonnam National University Department of Oral Pathology) | Hong Ran Choi
• 김옥준(School of Dentistry, Chonnam National University Department of Oral Pathology) | Ok Joon Kim correspondence