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635-nm 발광 다이오드가 임플란트 주위염 in vitro 모형에 미치는 효과 The Effects of 635-nm Light-Emitting Diode Irradiation in Peri-Implantitis in vitro Model

조민성, 최창우, Sandeep Karna, 임원봉, 김지선, 김상우, Zeng Hui, 전상미, 오희균, 김옥준, 최홍란
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대한구강악안면병리학회지
제36권 제4호 (2012.08)
pp.219-230
대한구강악안면병리학회 (Korean Academy Of Oral And Maxillofacial Pathology)
초록

Peri-implantitis (PI) is bacteria-induced inflammatory condition which affects the alveolar bone and soft tissue around implants and may result in the loss of supporting bone. Attenuation of the P. gingivalis lipopolysaccharide (LPS)-induced inflammatory response can be a new therapeutic approach in the treatment of PI. This study was conducted to evaluate the anti-inflammatory effect of 635-nm light-emitting diode (LED) irradiation over MG63 osteoblast-like cell. Scratch was made on MG63 cells with or without LPS, then 635-nm irradiated. The expression of the cyclooxygenase-2 (COX-2) proteins was evaluated with western blot. The production of the prostaglandin E2 (PGE2) and expression of the receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegerin (OPG) was measured with enzyme-linked immunoassay, and the cytokine profile was evaluated with the human inflammation antibody array. Wound closure effect presented in the cells treated with LPS was observed more significantly in the cells with 635-nm irradiation than the cells without irradiation. The 635-nm irradiaiton reduced LPS-induced expression of the COX-2 and production of the PGE2. Also, 635-nm irradiation affect the expression of RANKL, OPG, and proinflammatory cytokines. These results indicate that 635-nm irradiation could reduce the alveolar bone resorption induced by LPS stimulation through the inhibition of COX-2 expression and PGE2 production, the suppression of proinflammatory cytokine, and the modulation of RANKL/OPG balance in MG63 cells.

키워드
LEDperi-implantitis
목차
Ⅰ. Introduction
 Ⅱ. Material and Method
  1. Cell culture and chemicals
  2. Light source and irradiation
  3. In vitro wound healing assay
  4. Determination of PGE2
  5. Western-blot analysis
  6. Cytokine profiling
  7. Determination of OPG and RANKL
 Ⅲ. Results
  1. In vitro wound healing assay
  2. Express of COX-1, COX-2 and production ofPGE2
  3. Expression of RANKL and OPG
  4. Expression of cytokines
 Ⅳ. Discussion
 Ⅴ. Conclusion
 Ⅵ. References
저자
  • 조민성(School of Dentistry, Chonnam National University Department of Oral & Maxillofacial Surgery) | Min Sung Cho
  • 최창우(School of Dentistry, Chonnam National University Department of Oral Pathology) | Chang Woo Choi
  • Sandeep Karna(School of Dentistry, Chonnam National University Department of Oral Pathology)
  • 임원봉(School of Dentistry, Chonnam National University Department of Oral Pathology) | Won Bong Lim
  • 김지선(School of Dentistry, Chonnam National University Department of Oral & Maxillofacial Surgery) | Ji Sun Kim
  • 김상우(School of Dentistry, Chonnam National University Department of Oral & Maxillofacial Surgery) | Sang Woo Kim
  • Zeng Hui(School of Dentistry, Chonnam National University Department of Oral Pathology)
  • 전상미(School of Dentistry, Chonnam National University Department of Oral Pathology) | Sang Mi Jeon
  • 오희균(School of Dentistry, Chonnam National University Department of Oral & Maxillofacial Surgery) | Hee Kyun Oh
  • 김옥준(School of Dentistry, Chonnam National University Department of Oral & Maxillofacial Surgery) | Ok Joon Kim
  • 최홍란(School of Dentistry, Chonnam National University Department of Oral & Maxillofacial Surgery) | Hong Ran Choi correspondence