배양된 정상 사람조골세포의 증식과 분화에 BMP-2가 미치는 영향 The Effect of BMP-2 on Proliferation and Differentiation of Cultured Normal Human Osteoblast Cell
pp.263-268
Recently, extensive research has been performed in the field of orthopedic medicine to develop cell-based therapies for the restoration of injured bone tissue. But there has been rarely reported about rehabilitaton of oral and maxillofacial bone defect using self-derived osteoblasts. Normal human osteoblast cell(NHost) was previously established into marrow-derived human mesenchymal stem cells for their capacity to proliferate and differentiate into osteoblasts under various culture conditions. The purpose of this study was to examine proliferation and differentiation of NHosts effected by growth factors with ALP activity and RT-PCR. After NHosts were cultured under basal and osteogenic medium at 37℃ and 5% CO2, they were analyzed by ALP activity and RT-PCR. BMP-2 under osteogenic medium decreased growth rate of NHosts compared to under osteogenic medium. BMP-2 under osteogenic medium induced osteoblastic differentiation in NHosts by increased ALP activity. The differentiating capacity of NHosts under osteogenic medium showed that NHosts expressed higher mRNA expression levels of OSX and OCN, while that of RUNX2 decreased after BMP-2 treatment. It suggested that NHosts having characteristics of osteoprecursor cells might be more advanced in their osteogenesis development by BMP-2, making NHosts an interesting biological tool for treatment of skeletal defects and diseases of oral and maxillofacial bone.
II. Materials and Methods
1. Cell Culture
2. Growth Curve
3. RT-PCR Reaction for cDNA
4. Alkaline Phosphatase Activity Assay
III. Results
1. Growth Cutve of NHosts
2. Osteoblastic Phenotype of NHosts andRegulation by Osteogenic factors
3. Effects of BMP-2 on Cell Proliferation andDifferentiation of NHosts
IV. Discussion
V. References