KOREASCHOLAR

The dental infections have been increased due to the widespread overuse and exposure to antibiotics. One of the well-known pathogens is S. aureus The pathogenic properties of S. aureus is associated with the virulence gene. The objective of this study is to investigate the distribution of virulence gene of S. aureus which may contribute to the prevention and treatment of bacterial infections. 54 strains of S. aureus were separated from saliva taken from 129 outpatients diagnosed with periodontitis from Jun. to Dec., 2010 in Seoul. 44 (46.6%) and 13 (31.7%) strains of them were obtained from 88 and 41 outpatients from the S and E dental clinics, respectively. Then, the distribution of virulence gene and genetic diversity were analyzed with the PCR technique. The result of the S. aureus isolates possessed coagulase gene and showed six polymorphism types 390~470 bp (1.8%), 550~633 bp (3.7%), 630~714 bp (9.3%), 715~795 bp (20.4%), 796~876 bp (40.7%) and 877~957 bp(22.2%) due to variable numbers of tandem repeats present within the gene. In this study it will be anticipated that this study can contribute to establish efficiently the countermeasure about the prevention and treatment of antibiotic bacterial infections.

Ⅰ. 서론
 Ⅱ. 재료 및 방법
  1. 실험 검체
  2. 균 분리
  3. 균 동정
  4. 세균 Genomic DNA 추출
  5. 병원성 인자의 PCR 분석
  6. MRSA 분석시험
 Ⅲ. 결 과
  1. 시험균(S. aureus) 분리
  2. 병원성 유전자의 PCR 분석
 Ⅳ. 고 찰
 Ⅴ. 참고문헌

• 김연선(단국대학교 대학원 보건학과) | Yeon Sun Kim correspondence