KOREASCHOLAR

Cellular microenvironment is an essential issue for regulating epithelial characteristics through the alteration of intricate signaling pathways and intercellular communications in different cell types. Thus, microenvironment influences tumor initiation, progression, and metastasis. This study aimed to investigate the relationship between microenvironment and epithelial property in HPV16 E6/E7-immortalized human oral keratinocytes (IHOKs). To investigate characteristics of IHOK cultured in different media, two media were used, which included keratinocyte growth media (KGM), F-medium composed of 3:1 ratio of DMEM and F-12 (P media) supplemented with 10% FBS and 1% penicillin/streptomycin. Proliferative property and invasive and migratory activity were observed. As results, proliferating activities of IHOK in different culture condition were changed. Likewise, migratory and invasive activities were also different depending on media types. These results suggest that cellular microenvironment can affect modification of biological properties of epithelial cells.

Ⅰ. Introduction
 Ⅱ. Materials & Methods
  1. Reagents and Antibodies
  2. Cell culture
  3. Immunoblotting
  4. Cell proliferation assay
  5. Transwell invasion assay
  6. Transwell migration assay
  7. Three dimensional cell culture
  8. Gelatin zymography
  9. Statistical analysis
 Ⅲ. Results
  1. Modulation of cell morphology by cultureconditions
  2. Growth activity and expression of cell cycleregulating factors by different culture conditions
  3. Transwell migratory and invasive activity of cellscultured by different media
  4. Invasiveness of IHOKs in 3-dimension cellgrowth model and Zymography
 Ⅳ. Discussion
 Ⅴ. References

• 손화경(Oral Cancer Research Institute, Department of Oral Pathology, Yonsei University College of Dentistry) | Hwa Kyung Son
• 김진(Oral Cancer Research Institute, Department of Oral Pathology, Yonsei University College of Dentistry) | Jin Kim correspondence