HO-1 유도 Sappanchalcone의 당뇨병성 혈관 염증 억제 효능 Protective Effects of HO-1 Induced Sappanchalcone on Diabetic Vascular Inflammation
- 언어KOR
- URLhttp://db.koreascholar.com/Article/Detail/293331
pp.67-74
Sappanchalcone, a biologically active compound isolated from Caesalpinia sappan L., is reported to exert a variety of biological activities, such as neuroprotective, anti-inflammatory actions and inhibitory effect on antigen-induced beta hexosaminidase release. However, the vascular protective effects of this compound are not fully understood. The present study examined the effects of sappanchalcone in suppressing cell adhesion molecules expression in high glucose stimulated human umbilical vein endothelial cells (HUVEC). Sappanchalcone significantly decreased 25 mM high glucose-induced vascular cell adhesion molecule-1 (VCAM-1) and intracellular adhesion molecule-1 (ICAM-1) expression in a dose-dependent manner. Sappanchalcone also significantly inhibited the formation of intracellular reactive oxygen species (ROS). Furthermore, we found that the vascular protective effects of sappanchalcone were linked to the up-regulation of heme oxygenase-1 expression in HUVEC. The effects of sappanchalcone on the high glucose-induced expression of VCAM-1 and ICAM-1 were partially reversed by treatment of the cells with an inhibitor of HO-1, tin protoporphyrin IX (SnPP). Based on these results, these findings suggest that sappanchalcone-induced HO-1 expression plays a key role in the vascular protective effects of sappanchalcone in HUVEC.
II. 재료 및 방법
1. 실험재료
2. 시약 및 기기
3. HUVEC 세포배양 및 세포 독성 측정
4. ROS 측정
5. Western blotting 분석
6. 통계처리
III. 결과 및 고찰
1. 사람혈관 내피세포의 생존율에 대한 sappanchalcone의 영향
2. 고포도당에 의한 사람혈관 내피세포의 세포부착분자발현에 대한 sappanchalcone의 효과
3. 혈관 내피세포에서 Sappanchalcone의 HO-1 발현에 대한 영향
4. 혈관 내피세포에서 고포도당에 의해 유도된 ROS 생성에 대한 Sappanchalcone의 영향
IV. 결론
감사의 글
참고문헌