KOREASCHOLAR

Background. Vitamin K (VK) is a fat-soluble vitamin and is known to have anticancer activity in various cancer cell lines. However, there is no report on the anticancer effect of VK2 in mucoepidermoid carcinoma cells. Methods. The effects of VK2 on anti-proliferative and apoptotic activity were recognized by the trypan blue exclusion assay, 4'-6-diamidino-2-phenylindole (DAPI) staining and Western blot analysis. Results. The results showed that VK2 decreased cell viability and induced apoptotic programmed cell death in MC3 cells evidenced by the cleavages of caspase3 and PARP. VK2 treatment clearly increased Bak and truncated Bid (t-Bid) compared with the control treatment whereas it did not alter other Bcl-2 family members. Conclusions. Overall, our results suggest that VK2 can be a good apoptotic inducer accompanied by the increase in Bak and Bid protein. VK2 may be a potent target of anticancer drug candidate for the treatment of oral cancer.

Ⅰ. INTRODUCTION
 Ⅱ. MATERIALS AND METHODS
  1. Reagents and antibodies
  2. Cell Culture and Chemical Treatment
  3. Trypan blue exclusion assay
  4. DAPI staining
  5. Western blot analysis
  6. Statistical Analysis
 Ⅲ. RESULTS
  1. In vitro growth inhibitory effect of VK2 on MC3mucoepidermoid carcinoma cells
  2. Morphological detection of apoptosis of MC3cells treated with VK2
  3. Effects of VK2 on apoptosis and Bcl-2 familymembers in MC3 cells
 IV. DISCUSSION
 V. REFERENCES

• 장분실(Department of Oral Pathology, School of Dentistry, and Institute of Oral Bioscience Chonbuk National University) | Boonsil Jang
• 오세준(Department of Oral Pathology, School of Dentistry, and Institute of Oral Bioscience Chonbuk National University) | Sejun Oh
• 신지애(Department of Oral Pathology, School of Dentistry, and Institute of Oral Bioscience Chonbuk National University) | Ji Ae Shin
• 조남표(Department of Oral Pathology, School of Dentistry, and Institute of Oral Bioscience Chonbuk National University) | Nam Pyo Cho
• 조성대(Department of Oral Pathology, School of Dentistry, and Institute of Oral Bioscience Chonbuk National University) | Sung Dae Cho correspondence