KOREASCHOLAR

Arsenic (As) is a toxic element that easily taken up by plants root. Several toxic forms of As disrupt plant metabolism by a series of cellular alterations. In this study, we applied annealing control primer (ACP)-based reverse transcriptase PCR (polymerase chain reaction) technique to identify differentially expressed genes (DEGs) in alfalfa roots in response to As stress. Two-week-old alfalfa seedlings were exposed to As treatment for 6 hours. DEGs were screened from As treated samples using the ACP-based technique. A total of six DEGs including heat shock protein, HSP 23, plastocyanin-like domain protein162, thioredoxin H-type 1 protein, protein MKS1, and NAD(P)H dehydrogenase B2 were identified in alfalfa roots under As stress. These genes have putative functions in abiotic stress homeostasis, antioxidant activity, and plant defense. These identified genes would be useful to increase As tolerance in alfalfa plants.

Ⅰ. INTRODUCTION
 Ⅱ. MATERIALS AND METHODS
  1. Plant growth and treatments
  2. RNA Isolation and cDNA synthesis
  3. ACP-based GeneFishingTM reverse transcriptionchain reaction
  4. Cloning of gene and sequencing
 Ⅲ. RESULTS AND DISCUSSION
 Ⅳ. CONCLUSION
 Ⅴ. ACKNOWLEDGMENTS
 Ⅵ. REFERENCES

• Md Atikur Rahman(Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan, 330-801, Republic of Korea)
• Sang-Hoon Lee(Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan, 330-801, Republic of Korea)
• Ki-Yong Kim(Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan, 330-801, Republic of Korea)
• Hyung Soo Park(Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan, 330-801, Republic of Korea)
• Tae Young Hwang(Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan, 330-801, Republic of Korea)
• Gi Jun Choi(Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan, 330-801, Republic of Korea)
• Ki-Won Lee(Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan, 330-801, Republic of Korea) Corresponding author