KOREASCHOLAR

Gene Induction to Apoptosis Using ENDO G in Tumor Cells Instead of CAD or AIF and Prediction for Function of Oncogenes by Microarray Analysis

SungGa Lee, Won-Bong Lim, In-Ae Kim, Min-A Jung, Ok-Joon Kim, Hong-Ran Choi
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  • URLhttp://db.koreascholar.com/Article/Detail/324564
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대한구강악안면병리학회지
제31권 제5호 (2007.10)
p.303
대한구강악안면병리학회 (Korean Academy Of Oral And Maxillofacial Pathology)
초록

Glycyrrhiza ura lensis (Leguminosae) has long been known as an ant i-i nflammatory agent for gastric 띠cers ， arthri t is, and rheumaLism. 1'he f1avonoid glycyr ol isolated from Glycyrrhiza uralensis dramatically inhibits phorbol ester (PMA)-induced NF-kB-dependent transcriptional activity, as determined by luciferase activity in HEK293T cells To in vestigate the global gene expression profiling by glycyrol, we performed high-density oligonucleotide microarrays, Our microarray analysis showed that gl ycY I 이 inhi bited phorbol ester-induced NF-kB-dependent transcriptional activity in inflarnmatory-related gene expression, RT-PCR analysis based on microarry data showed that NF-kB-dependent genes such as CCL2‘ CCL7. CD44, and HSPB8 in addition to NF-kB itself were significantly down-regulated by glycyrol Treatment with glycyrol inhibited ]-kB degradation induced by PMA, suggesting that glycyrol has a potential anti-in flammatory activity by modulating NF-kB-dependent pathways. Also, the microarray data showed that glycyr이 appears to induce gene expression related to p53-dependent apoptosis t hrough ENDO G instead of CAD/DFF and AlF/PDCD8 as down stream apoptosis factor in HEK293T tumor cell s and to induce an added function as oncogenes in connection with apoptoslS

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저자
  • SungGa Lee(Department of Oral Path이。gy， 2nd stage of brain korea 21 for school of dentistry, Dental Science Research Institute, Chonnam National University)
  • Won-Bong Lim(Department of Oral Path이。gy， 2nd stage of brain korea 21 for school of dentistry, Dental Science Research Institute, Chonnam National University)
  • In-Ae Kim(Department of Oral Path이。gy， 2nd stage of brain korea 21 for school of dentistry, Dental Science Research Institute, Chonnam National University)
  • Min-A Jung(Department of Oral Path이。gy， 2nd stage of brain korea 21 for school of dentistry, Dental Science Research Institute, Chonnam National University)
  • Ok-Joon Kim(Department of Oral Path이。gy， 2nd stage of brain korea 21 for school of dentistry, Dental Science Research Institute, Chonnam National University)
  • Hong-Ran Choi(Department of Oral Path이。gy， 2nd stage of brain korea 21 for school of dentistry, Dental Science Research Institute, Chonnam National University)