Human Umbilical Cord Blood-Derived Stem Cells: Present and Future Perspectives
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3BK21 program for Veterinary Science, College of Veterinary Medicine‘ Seoul National University. Seoul. Korea Human Co1'd blood has been used for the alternatives of bone marrow transplantation for more 10 years. Recently Mesenchymal s tem cell s , ES-like cells and endothelial stem cells has been successfuly isolated from huam co1'd blood Presentl y. it has been reported that a bout 70 incurable ans tractible di sease was possibly cured by umbili cal cord blood-deri ved s tem cells in the clinit;al test s‘ However‘ isolation and expansion of s tem cells from human umbilical cord blood(UCB) have been very difficult and an obstrucle for the clinical use This study showed that effi cient s iolat iona and expans ion of mesenchymal stem cells from UCB Full term UCB samples were obtained from the umbi lical vein after vaginal deli ve ry with the informed consent 0 1' the mothe1' approved by Borame Hospital Institutional Review Broad (IRB). And a lso. t his work was also a pproved by Seoul National University IRB. Recently, we isolated a population of s tem cells from human corcl bloocl (UCB)‘ which expressed embryo stage specific maker. SSEA-4. ancl the multi-potential stem cell marker‘ 。c t4 And we have sucessfully developed culture methods to expand ancl subculture these cells up to 1.000 billion from one single clone. Subsequently. we were a ble to transclifferente theses stem cells into insulin- producing is let- like structures. which co-express in sulin andC-pepticle, adipocyte, neuron‘ bone and cartilage. In acldition. the isola tion rate of MSC from UCB is about 70 % from the cord blood units. This isolation rate were not affected by maternal ages. the sex of baby, isolation time from the deli very. for example. 12 hrs. 24 hrs ‘ even 48 hrs from delivery Taken together. these findings might have a s ignificant potential to aclvance human UCB clerivecl stem- cell -basecl ther apeutics fOI' clinical use in near future