KOREASCHOLAR

Epithelial-mesenchymal transition(EMT) plays a pivotal role in the convers ion of earl y s tage tumors into invasive malignancies‘ and has been shown to be regulated hy the transcri ptional factor. Snail. Recent ly‘ actlvatlon of the phosphatidylinositol 3' kinase (PI3K)/따<:T axis is emerging as a centra l feature of EMT‘ However. it is unclear whether the phosphorylation of AKT regulate the expl'ession of s nail in ora l cancer cell underwent EMT. T。 investigate a role of p-AKT in EMT, we assessed the effects of inhibi ting p-AK1' activity in oral squamous can cer cells(KOSCC-25B) using PIAs, structurally modified phosphatidyli nositol ether lipid analogues(P1As) . PIAs de creased phosphorylation of c-Jun N-terrninal Kinase(JNK) and increased phosphorylation of glycogen synthase kinase 3beta(GSK-3beta). Inhibition of p-AKT ir빼ce d down regulation of Snail and Twist. but Sip1 regulated independent of p-AKT inhibition. Also inhibi tion of p-AKT dec reased cell migration and invas ion. Therefore our results implicate that p-AKT may contribute to the translocalization of sna il in the EMT associated with canceJ cell rnigration and invasion

• Kyoung Ok Hong(Depf. of Oral Pafhology, School of Denfisfry, Seoul Nafional University)
• Ji Soo Hong(Depf. of Oral Pafhology, School of Denfisfry, Seoul Nafional University)
• Jae ll Lee(Depf. of Oral Pafhology, School of Denfisfry, Seoul Nafional University)
• Sam Pyo Hong(Depf. of Oral Pafhology, School of Denfisfry, Seoul Nafional University)
• Seong Doo Hong(Depf. of Oral Pafhology, School of Denfisfry, Seoul Nafional University)