KOREASCHOLAR

Substance P Regulates Macrophage Inflammatory Protein 3a /CCl20 with Heme Oxygenase-1 in Human Periodontal Ligament Cells

Sun Kyung Lee, Sung Hee Pi, Sung Hyun Kim, Kyung San Min, Hwa Jeong Lee, Hoon Sang Chang, Kyung Hwa Kang, Hyung Ryong Kim, Hong ln Shin, Suk Keun Lee, Eun Cheol Kim
  • 언어ENG
  • URLhttp://db.koreascholar.com/Article/Detail/324630
대한구강악안면병리학회지
제31권 제2호 (2007.04)
p.127
대한구강악안면병리학회 (Korean Academy Of Oral And Maxillofacial Pathology)
초록

Al t hough substance P(SP) , a potent pro- inflammatory peptide, is involved in inflammation and immune responses‘ t he eff'ect of SP on t he expression of macrophage inflammatory protein 3a (MIP- 3α CCL20) in periodontal liga ment(PDL) cell s a re unknown, Equally as enigmatic is the link between SP, t he stress protein heme oxygenase- l(HO-l) ‘ and CCL20 procluction, We investigated whether SP induces the release of chemokine CCL20 from immortal ized PDL(IPDL) ceJJ s‘ and fur ther c l a꺼 SP mediated pathways, We also examined the relationship between HO-l a ncl CCL20 by t reating PDL cells with SP, Incubating IPDL cells with SP increased expression of CCL20 mRNA a nd CCL20 protein in a dose-time dependent manner Highly selective p38 and ERKl/2 inhibitors abrogated SP-induced expression of CCL20 in IPDL cell s, SP is a lso responsible for ini t iating phosphorylation of I/C B, degradation of Iκ B‘ ancl activat ion of NF'-/C B, SP induced expression of HO-l in both a concentration- and time-dependent man nel ‘ and CCL20 refl ected s imilar patterns, The inductive effects o[ SP on HO- l and CCL20 wer e enhanced by HO- j inducer hemin and the membrane-permeable cGMP analog 8-bromo-cGMP, Conversely, this pathway was inJübited by t he 1-10난 inhi bitor zinc protoporphyrin IX(ZnPP IX) and the selective inl뼈itor of guanylate cyc1ase‘ lH-[l , 2, 4Joxad iazole[4‘ 3-aJquinoxal in-l-one (ODQ) , We report herein the pathway that connects SP along with other modulators 。f neuroimmunoregulationto the induction of HO-l and t he inflammatory mediator MIP-3a /CCL20 in IPDL cell s‘ which play an important role in the development 01' periodontitis or inflamrnation during orthodontic tooth movem

저자
  • Sun Kyung Lee(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)
  • Sung Hee Pi(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)
  • Sung Hyun Kim(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)
  • Kyung San Min(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)
  • Hwa Jeong Lee(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)
  • Hoon Sang Chang(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)
  • Kyung Hwa Kang(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)
  • Hyung Ryong Kim(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)
  • Hong ln Shin(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)
  • Suk Keun Lee(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)
  • Eun Cheol Kim(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)