KOREASCHOLAR

LED Irradiation Decreases CoCb-induced Apoptosis In Human SH-SY5Y Neuroblastoma Cells : Signaling Mechanisms and Therapeutic Opportu

Jae Hyuck Yang, Youn Young Jang, Kwon Jung, Mi Kyeong Ko, Mi Sun Jang, Eun Byul Kook, Chae Kwang Lim, Won Bong Lim, Jin Soo Park, Ok Joon Kim, Hong Ran Choi
  • 언어ENG
  • URLhttp://db.koreascholar.com/Article/Detail/324674
대한구강악안면병리학회지
제29권 제5호 (2005.10)
p.374
대한구강악안면병리학회 (Korean Academy Of Oral And Maxillofacial Pathology)
초록

The purpose 01' pl'esent study was to examine the molecular events in apoptosis by CoCl2, mimicking hypoxic cond ition and recovering effects by LED ir l'adiation on Human SH-SY5Y neuroblastoma cells The SOUl'ce 0 1' light for ir l'adiation was a continuous-wave LED emitting at a wavelenl양h of 590 nm, and manufactured that ene rgy density was 5 mW!cm2 on sample surface, After ir l'adiation, cell viabi lity was measured with BrdU , cell morphol ogy was examined with Diff- Quik staining, cell signaling was monitored with various apoptosis-related molecules using RNase Pl'otection Assay(RPA) , W11en treated with CoC12, apoptotic induction was found in the SH-SY5Y cells in a concentration-dependent and time-dependent manner , Diff-Quik s taining was revealed that DNA fragmentation re presented apoptosis was examined in CoC12-tl'eated group, Moreover, RPA assay of SH-SY5Y cclls lIs ing val'iolls apoptosis-related molecllles showed that the apoptotic cell population was mcreased J-loweve. there was sorne signifïcant change in LED irradiatied cells aftel' treatement of CoC12 The main mechanism for Lhese a poptosis appearecl to be mito c hondriεt - m ecliated pathway, such as cytochrome- c‘ caspase-9, caspase-3, pro-apototic protein ßax, anti-apototic protein Bcl-2, and death receptor• mediated pathway, such as Fas, cas pase- 8, a ncl TNFRl These results demonstrate that CoCI2 induce apoptosis in SH-SY5Y via different dual apop tosis pathway through death receptor pathway as well as mitochondria- dependent pathway and LED irradiation can recl llces the CoCl2-induced apoptosis by blocking their internal signaling pathway

키워드
저자
  • Jae Hyuck Yang(Dental Research Institlute, Depatment of Oral Pathology, School of Dentistry, Chonnam National University)
  • Youn Young Jang(Dental Research Institlute, Depatment of Oral Pathology, School of Dentistry, Chonnam National University)
  • Kwon Jung(Dental Research Institlute, Depatment of Oral Pathology, School of Dentistry, Chonnam National University)
  • Mi Kyeong Ko(Dental Research Institlute, Depatment of Oral Pathology, School of Dentistry, Chonnam National University)
  • Mi Sun Jang(Dental Research Institlute, Depatment of Oral Pathology, School of Dentistry, Chonnam National University)
  • Eun Byul Kook(Dental Research Institlute, Depatment of Oral Pathology, School of Dentistry, Chonnam National University)
  • Chae Kwang Lim(Dental Research Institlute, Depatment of Oral Pathology, School of Dentistry, Chonnam National University)
  • Won Bong Lim(Bíophoton, 705, Business Incubation Center, Chonnam Natíonal University)
  • Jin Soo Park(Bíophoton, 705, Business Incubation Center, Chonnam Natíonal University)
  • Ok Joon Kim(Dental Research Institlute, Depatment of Oral Pathology, School of Dentistry, Chonnam National University)
  • Hong Ran Choi(Dental Research Institlute, Depatment of Oral Pathology, School of Dentistry, Chonnam National University)