KOREASCHOLAR

Heme Oxygenase-1 Mediates Cytoprotection Against Nitric Oxideinduced Cytotoxicity via the cGMP Pathway in Human Pulp Cells

Kyung San Min, Young Hee Hwang, Hyun Jin Ju, Hoon Sang Chang, Kyung Hwa Kang, Sung Hee pi, Sun Kyung Lee, Eun Cheol Kim
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대한구강악안면병리학회지
제29권 제5호 (2005.10)
p.374
대한구강악안면병리학회 (Korean Academy Of Oral And Maxillofacial Pathology)
초록

This study was to taken to demonstrate the effects of exogenous nitric oxide(NO) on hu rnan pu lp cell s ‘ In volvement of cyclic 3’, 5' -monophosphate(cGMP) in p버 paJ protection induced by herne oxygenase-l (J-lO-l) against NO-induced cytotoxicity , By use of Western blotting and cell viabi lity assay, we have examined the cytotoxicity and J-lO-l induction in pulp cells that were treated with NO donor ‘ S-nitroso-N-acetyl-D, L-penici 1 lamine(SNAP) , We have assessed wheathel' HQ--l contributes the cytoprotective effect against the cytotoxicity caused by NO, and inves tigated the l'elationship between HO-l and cGMP in the s ignaling pathway, SNAP decreased cell via bility but in creased HO-l expl'ession in a concentl'ation- and time一dependent manner in hurnan pu lp cells NO-induced cyto toxicity was inhibited in the presence of the hemin(inducer of HO-l) , whel'eas was en hanced in the pl'esence zinc protoporphyrin IX(ZnPP IX, HO-l inhibitor), thus Lhe NO-induced cytoLoxicity was cOl'related with HO- l expression. R‘ etreatment with a rnemhrane-permeable cGMP analog, 8-bromo-cGMP, restored cell death and enhanced the HO-l protein expression induced by SNAP, ln contrast‘ inhibition of guanylate cyclase by lI-l -[1,2,4] ox adiazole[ 4,3 口]quinoxalin-l-one(ODQ) pretreated pulp cells to 1 mM SNAP resulting in marked cytotoxicity , These findings , demonstrating a link between J-lO-l, regulated thl'ough the cGMP system and NO-induced cytotox.icity in huma띠 p버 p ceJls , suggesti ng a protective 1'ole of HO-l in pulp infl ammatory disease

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저자
  • Kyung San Min(Department of Opeatíve Dentístry, Department of Oral & Maxillolacíal Pathology, College of Dentistry, Wonkwang University)
  • Young Hee Hwang(Department of Opeatíve Dentístry, Department of Oral & Maxillolacíal Pathology, College of Dentistry, Wonkwang University)
  • Hyun Jin Ju(Department of Opeatíve Dentístry, Department of Oral & Maxillolacíal Pathology, College of Dentistry, Wonkwang University)
  • Hoon Sang Chang(Department of Opeatíve Dentístry, Department of Oral & Maxillolacíal Pathology, College of Dentistry, Wonkwang University)
  • Kyung Hwa Kang(Department of Opeatíve Dentístry, Department of Oral & Maxillolacíal Pathology, College of Dentistry, Wonkwang University)
  • Sung Hee pi(Department of Opeatíve Dentístry, Department of Oral & Maxillolacíal Pathology, College of Dentistry, Wonkwang University)
  • Sun Kyung Lee(Department of Opeatíve Dentístry, Department of Oral & Maxillolacíal Pathology, College of Dentistry, Wonkwang University)
  • Eun Cheol Kim(Department of Opeatíve Dentístry, Department of Oral & Maxillolacíal Pathology, College of Dentistry, Wonkwang University)