KOREASCHOLAR

Multiplex-PCR 및 Plasmid Profiling에 의한 Salmonella Typhimurium의 분자생물학적 특성 Molecular Characterization of Multidrug-Resistance Salmonella Typhimurium by Multiplex-PCR and Plasmid Profiling

정윤태, 이영기, 오영희
  • 언어KOR
  • URLhttp://db.koreascholar.com/Article/Detail/324713
대한구강악안면병리학회지
제29권 제3호 (2005.06)
pp.225-230
대한구강악안면병리학회 (Korean Academy Of Oral And Maxillofacial Pathology)
초록

In order to investigate the MDR gene by plasmid profile, multiplex-PCR and PFGE analysis, it was examined 20 Salmonella Typhimurium isolated from patients stool from 1999 to 2002. The plasmid profile analysis was shown one to five kinds of plasmid in 600bp~150Kb sized. Especially, PT104 isolates obtained has 3 plasmid identically. According to the result of multiplex-PCR, all PT104 isolates amplified only tet(G) and pse1, but aadA2 was not specialized. PFGE analysis of the fragments restricted by XbaⅠ evidently discriminated plasmid DNA among phage types.

저자
  • 정윤태(서울특별시 보건환경연구원) | Yoon Tae Chung
  • 이영기(단국대학교 교양학부 초빙교수) | Young Ki Lee
  • 오영희(서울특별시 보건환경연구원) | Young Hee Oh