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구강 편평세포암종에서의 Microsatellite Instability Microsatellite instability in oral squamous cell carcinomas

이국엽, 손현진, 최철영, 조남표
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  • URLhttp://db.koreascholar.com/Article/Detail/324743
대한구강악안면병리학회지
제28권 제3호 (2004.09)
pp.177-188
대한구강악안면병리학회 (Korean Academy Of Oral And Maxillofacial Pathology)
초록

To cletermine the role of mismatch repair in the clevelopment of oral squamolls cell carcinomas (OSCCs) , the prevalence of microsatellite instability (MSI), expression of I뼈LH1 ancll띠SH2 , ancl hypennethylation of I뻐LH1 ancl hMSH2 were explorecl. Bya panel of five markers (BAT25, BAT26 , 02S123, 05S346, ancl 017~‘250 , the so-callecl Bethescla markers) for screening of MSI, MSI was observecl in 5 of the 15 sqllamolls cell carcinomas (33.3%). As MSI is callsecl by the clysfllnction of MMR genes, this stucly examinecl the methylation status of CpG sites in the hMLH 1 ancl hMSH2 promoters ancl the expression hMLH1 ancl hMSH2. Becallse of inappr이)riate efficiency of fonnalin-frxecl ancl paraffin-embeclclecl samples for methylation-specific PCR (MS-PCR) of hMLH1 ancl hMSH2, the role of promoter hypelmethylation 띠 the clevelopment of MSI ancl expression of hMLH1 ancl hMSH2. meùlylation was failecl to clefìne. However, loss of nllclear staining of 1ψIILH 1 ancl hMSH2 were seen in nine (69.2%) ancl four (30.7%) of 13 OSCCs, while four ancl fìve all corresponcling normal epiùlelial tisslles showecl positive nllclear stι ining ofl마ILH1 and hMSH2. These data suggest that MSI anclloss of expression of hMLH1 ancl hMSH2 play a role in the carcinc핑enesis of oral sqllamolls cell carcinomas thollgh the role of promoter hypermethylation of hMLH1 ancl hMSH2 in MSI and their expresslon IS lIncertam

저자
  • 이국엽(전북대학교 치과대학 구강악안면외과학교실) | Kuk-Yeob Lee
  • 손현진(전북대학교 의과대학 병리학교실) | Hyun-Jin Son
  • 최철영(전북대학교 치과대학 구강병리학교실 및 구강생체과학연구소) | Cheol-Young Choi
  • 조남표(전북대학교 치과대학 구강병리학교실 및 구강생체과학연구소) | Nam Pyo Cho