This research focused on Road Bike synchronized with the virtual reality software. To clarify the effectiveness of exercise using a indoor Road Bike synchronized with the virtual reality software in this research, the changes of stress index were observed between synchronized status of Road Bike hardware and virtual reality software and not synchronized status. The virtual reality Road Bike which is synchronized with 3D virtual reality environment and mechanically works the same way with real bike allows a users not only to increase the effectiveness of exercise but also to reduce significantly the boredom compare to traditional indoor bike. The prototype of virtual realty Road Bike has been completed, detailed research will be conducted in the future to increase a user‘s safety and provide a more similar experience with real Road Bike for adapting various industry fields especially in the game industry.