In-cylinder flows in a motored 3.5L four-valve SI engine are investigated quantitatively using three-dimensional LDV system to determine how intake system affects the flow field. For this study, the same engine head, cylinder, and piston are used. The purpose of this work is to develop quantitative methods which correlate in-cylinder flows to engine performance. The LDV results reveal that collision regions and zones around vortices can be traced as the origins of turbulent kinetic energy. High levels of turbulent kinetic energy are found in regions of high shear flow, attributed to the collisions of intake flows. These specific results support the more general conclusion that the inlet conditions play the dominant role in the generation of the turbulence fields during the intake stroke.