생물반응기를 이용한 프리지아‘Shiny Gold’구경 기내 급속 증식 Rapid Mass Propagation of Freesia‘Shiny Gold’Corms Using a Bioreactor
pp.208-215
Because freesia is a crop that is typically vegetatively propagated from offsets, a degradation in its genetic character or an onset of after successive culturing over a long period. To produce virus-free freesia corms, apical meristem culture and rapid mass propagation must be established. This study aimed to establish the rapid mass production of in vitro freesia corms using a bioreactor. Shoots (12 – 15 cm in height) obtained from tissue culture were used as the plant material in a balloon-type bubble bioreactor in which medium strength, sucrose concentration, inoculation plant volume, and medium exchange method were determined. Medium strengths tested included full strength (1), 1/2, and 1/4 strength MS medium; sucrose concentration 9% 12%, and 15%, and inoculation plant volumes were tested at 40, 80, 120, and 160 shoots/1L medium. MS medium was supplemented with a 1/2 volume of the initial volume or the medium was completely exchanged with a new medium or not exchanged at all throughout the experiment. Results indicated the best growth of freesia corms at 1/2 strength medium, 9% sucrose, 120 shoots/L medium, with complete medium exchange 4 weeks after initiating the experiment. Under these culturing conditions, corms with 0.5 g fresh weight, 0.15 g d ry w eight, 1 3 mm h eight, a nd 0 .9 mm diaeter were harvested from the bioreactor within only 6 weeks, which were large enough to be planted in field. Thus, our results indicated that the cost and period for the production of freesia corms in vitro can be markedly decreased.
재료 및 방법
식물재료 및 생물 반응기
구경 비대를 위한 배양 조건
결과 및 고찰
MS배지 농도, sucrose농도 및 치상 신초수가 구경비대에 미치는 영향
배지교체 방법이 기내 구경비대에 미치는 영향
References