Fluid motion within the internal combustion engine cylinder plays a major role in controlling the fuel/air mixing and combustion processes in spark-ignition engines, and the combustion processes in compressionignition engines. In-cylinder flow is quite unstable and varies from one cycle to another. Various methods of in-cylinder flow measurement and fuel/air mixing characterization have been developed during the past few decades. In particular, laser based flow diagnostic techniques have been utilized for this purpose. This study will focus on the quantification of spark-ignition engine in-cylinder flow using the laser based flow diagnostic techniques. The measurement methods, including high speed flow visualization and laser Doppler velocimetry (LDV), will be discussed.