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9. Cold, Salt and Heat-induced Genes Identified in Italian Ryegrass (cv. Kowinearly) Leaves using Annealing Control Primer (ACP) Based Approach

Md. Atikur Rahman, Sang-Hoon Lee, Hee Chung Ji, Gi Jun Choi, Yowook Song, Tea Young Hwang, Jung Ye Lee, Ki-Won Lee
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  • URLhttp://db.koreascholar.com/Article/Detail/360578
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한국초지조사료학회 학술대회논문집
2018년도 한국초지조사료학회 한국축산환경학회 공동심포지엄 및 제55회 학술발표회 (2018.09)
pp.156-157
한국초지조사료학회 (The Korean Society of Grassland and Forage Science)
초록

Cold, salt and heat are most critical factors that restrict full genetic potential, growth and development of crops worldwide.. In this study, we applied an annealing control primer (ACP) based GeneFishing approach to identify differentially expressed genes (DEGs) in annual ryegrass (cv. Kowinearly) leaves under cold, salt and heat stresses. Two-week-old seedlings were exposed to cold (4°C), salt (NaCl 200 mM) and heat (42 °C) treatments for 6 h. A total 8 differentially expressed genes were isolated form ryegrass leaves. These genes were sequenced then identified and validated form National Center for Biotechnology Information (NCBI) database. We identified several promising genes encoding light harvesting chlorophyll a/b binding protein, alpha-glactosidase b, chromosome 3B, elongation factor 1-alpha, FLbaf106f03, complete genome, translation initiation factor SUI1, and glyceraldehyde-3-phosphate dehydrogenase. These genes were potentially involved in photosynthesis, plant development, protein synthesis and abiotic stress tolerance in plants. These genes might be useful for the enhancement of abiotic stress tolerance in fodder crops along with crop improvement under unfavorable environmental conditions.

키워드
Abiotic stressGeneRyegrassForage
저자
  • Md. Atikur Rahman(Grassland and Forage division, National Institute of Animal Science, Rural Development Administration)
  • Sang-Hoon Lee(Grassland and Forage division, National Institute of Animal Science, Rural Development Administration)
  • Hee Chung Ji(Grassland and Forage division, National Institute of Animal Science, Rural Development Administration)
  • Gi Jun Choi(Grassland and Forage division, National Institute of Animal Science, Rural Development Administration)
  • Yowook Song(Grassland and Forage division, National Institute of Animal Science, Rural Development Administration)
  • Tea Young Hwang(Grassland and Forage division, National Institute of Animal Science, Rural Development Administration)
  • Jung Ye Lee(Grassland and Forage division, National Institute of Animal Science, Rural Development Administration)
  • Ki-Won Lee(Grassland and Forage division, National Institute of Animal Science, Rural Development Administration)