KOREASCHOLAR

Glutathione S-transferase (GST) is a key gene involved in multiple stress tolerance in all living organisms, though it is still to be disclosed the gene function in teff grass [Eragrostis tef (Zucc.)Trotter].The objectives of this study were to clone and molecular characterization of GST gene in teff grass. We characterized GST1 from teff grass (EtGST1), it composed of a 645-bp open reading frame (ORF) that encoded 195 amino acid residue. Further, we transformed EtGST1 in E.coli BL21 (DE3) cells. This recombinant EtGST1 in E.coli BL21(DE3) induced at 37°C temperature. In addition, Growth of cells overexpressing EtGST1 rapidly increased in the presence of polyethylene glycol (5%), heat (46°C), NaCl (0.6%), and arsenic (1 mM) than that of cells harboring an empty vector. These results suggest that EtGST1 would be suitable candidate for improving tolerance in forages and/or grasses species against multiple abiotic stresses.

• Ki-Won Lee(Grassland and Forage division, National Institute of Animal Science, Rural Development Administration)
• Md. Atikur Rahman(Grassland and Forage division, National Institute of Animal Science, Rural Development Administration)
• Hee Chung Ji(Grassland and Forage division, National Institute of Animal Science, Rural Development Administration)
• Gi Jun Choi(Grassland and Forage division, National Institute of Animal Science, Rural Development Administration)
• Ji Hye Kim(Grassland and Forage division, National Institute of Animal Science, Rural Development Administration)
• Tea Young Hwang(Grassland and Forage division, National Institute of Animal Science, Rural Development Administration)
• Sang-Hoon Lee(Grassland and Forage division, National Institute of Animal Science, Rural Development Administration)