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생약 복합 추출물의 인간 골수 유래 중간엽 줄기세포에서의 골 분화에 미치는 영향 The Effect of Natural-Herb Mixture Extract on Osteogenic Differentiation in Human Mesenchymal Stem cells

김다솔, 성기수, 박민성, 김미경, 박경은, 곽연주, 윤금정, 김형준, 배문경, 배수경, 장혜옥
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대한구강악안면병리학회지
제43권 제5호 (2019.10)
pp.171-178
대한구강악안면병리학회 (Korean Academy Of Oral And Maxillofacial Pathology)
초록

Herbal medicine has been the basis for medical treatments through much of human history, and such traditional medicine is still widely practiced today. Modern medicine makes use of many plant-derived compounds as the basis for pharmaceutical drugs. In traditionally, Achyranthes aspera, Safflower (Carthamus tinctorius) seed and Acanthopanax senticosus have been used for the treatment and prevention of bone-related diseases. In this study, we investigated the pharmacological effect of mixture of Achyranthes aspera, Safflower (Carthamus tinctorius) seed and Acanthopanax senticosus and the other herbs. Two types of enzymes were used to enhance the extraction components of amino acid, mineral content, free sugar, and flavor recovery in extracting natural herbal mixtures(NME). We evaluated regulation of osteogenic differentiation in human bone marrow mesenchymal stem cells using alkaline phosphatase staining, alizarin red S staining and RT-PCR. The CCK-8 assay indicated that NME had no cytotoxicity but increased cell survival. In addition, NME promoted the mineralization and expression of osteogenic differention marker genes in human bone marrow mesenchymal stem cells. Therefore, NME has an effect of promoting proliferation and osteogenic differentiation of human mesenchymal stem cell.

키워드
NME extractHuman bone-marrow mesenchymal stem cellsOsteogenic differentiationAPA
목차

Ⅰ. INTRODUCTION
Ⅱ. MATERIALS and METHODS
    1. 실험재료
    2. 세포배양
    3. 세포의 분화
    4. 세포 생존률 측정
    5. Alkaline phosphatase (ALP) 염색
    6. Alizarin Red S 염색법
    7. RNA isolation와 cDNA 합성
    8. RT-PCR
    9. 통계 처리
Ⅲ. RESULTS
    1. NME 농도에 따른 인간 골수 유래 중간엽 줄기세포 증식 정도 확인
    2. NME 처리에 의한 ALP 활성 확인
    3. NME 처리에 의한 미네랄 형성정도 확인
    4. NME 처리에 의한 인간 골수 유래 중간엽 줄기세포의 골 분화 마커의 발현
Ⅳ. DISCUSSION
REFERENCES
저자
  • 김다솔(부산대학교 치의학전문대학원 치과약리학교실, 치주질환네트워크신호센터(MRC)) | Da-Sol Kim (Department of Dental Pharmacology, School of Dentistry, Pusan National University, Periodontal Disease Signaling Network Research Center (MRC))
  • 성기수(부산대학교 치의학전문대학원 치과약리학교실) | Ki-Soo Seong (Department of Dental Pharmacology, School of Dentistry, Pusan National University)
  • 박민성(부산대학교 치의학전문대학원 치과약리학교실) | Min-Sung Park (Department of Dental Pharmacology, School of Dentistry, Pusan National University)
  • 김미경(부산대학교 치의학전문대학원 치과약리학교실) | Mi-Kyoung Kim (Department of Dental Pharmacology, School of Dentistry, Pusan National University)
  • 박경은(부산대학교 치의학전문대학원 치과약리학교실) | Kyoung-Eun Park (Department of Dental Pharmacology, School of Dentistry, Pusan National University)
  • 곽연주((주)가고파 힐링푸드) | Yeon-Ju Kwak (Research Institute of GH BioFarm, Agricultural Corporation GAGOPA-HEALING FOOD, Changwon)
  • 윤금정((주)가고파 힐링푸드) | Geum-Joung Youn (Research Institute of GH BioFarm, Agricultural Corporation GAGOPA-HEALING FOOD, Changwon)
  • 김형준(부산대학교 치의학전문대학원 구강생리학교실, 치주질환네트워크신호센터(MRC)) | Hyung-Joon Kim (Department of Oral Physiology, School of Dentistry, Pusan National, Periodontal Disease Signaling Network Research Center (MRC))
  • 배문경(부산대학교 치의학전문대학원 구강생리학교실, 치주질환네트워크신호센터(MRC)) | Moon-Kyoung bae (Department of Oral Physiology, School of Dentistry, Pusan National, Periodontal Disease Signaling Network Research Center (MRC))
  • 배수경(부산대학교 치의학전문대학원 치과약리학교실, 치주질환네트워크신호센터(MRC)) | Soo-Kyung Bae (Department of Dental Pharmacology, School of Dentistry, Pusan National University, Periodontal Disease Signaling Network Research Center (MRC))
  • 장혜옥(부산대학교 치의학전문대학원 치과약리학교실) | Hye-Ock Jang (Department of Dental Pharmacology, School of Dentistry, Pusan National University) Correspondence