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저산소성 조건에 노출된 구강암세포의 상피간엽전환관련 유전자 발현 프로파일 Epithelial-Mesenchymal Transition-Related Gene-Expression Profile in Hypoxic Oral Cancer Cells

박대근, 김소라, 우복희, 이지혜, 박혜련
  • 언어ENG
  • URLhttp://db.koreascholar.com/Article/Detail/386469
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대한구강악안면병리학회지
제43권 제6호 (2019.12)
pp.237-243
대한구강악안면병리학회 (Korean Academy Of Oral And Maxillofacial Pathology)
초록

Hypoxia is one of the most common features of cancer. It is also associated with cancer progression and the acquisition of aggressiveness, which includes invasion and metastasis. Oral squamous cell carcinoma accounts for 90% of all oral cancers, and its 5-year survival rate is about 50%. Despite various attempts and trials, its prognosis has not improved. Among numerous adverse prognostic factors, hypoxia is suspected as one of the most important factors, as it increases the aggressiveness of oral cancer cells. We attempted to observe the effect of hypoxia on the expression of epithelial-mesenchymal transition markers in oral cancer cells. We analyzed and compared both the mRNA and protein expression levels of epithelial-mesenchymal markers using qRT-PCR and western blotting in both normoxic and hypoxic YD10B oral squamous cell carcinoma cells. Eighty-six genes were analyzed through real-time PCR using commercial microarray plates, performed in triplicate. Among the 86 genes, the expression of 24 were increased (≥ 2 fold) by hypoxia, while that of three genes was decreased (≥ 2 fold). Hypoxia significantly affects epithelial-mesenchymal transition-related genes. Further studies on the regulation of these genes may help to develop more efficient therapeutic modalities for oral cancer and to improve prognosis of oral cancer patients.

키워드
HypoxiaOral cancerEpithelial-mesenchymal transitionTCF-4
목차

Ⅰ. INTRODUCTION
Ⅱ. MATERIALS & METHODS
    1. Cell culture and hypoxic treatment
    2. Cell viability assay
    3. Gene expression by the EMT PCR array
    4. Western blot analysis
    5. Transient Transfections and luciferase assay.
    6. Statistical analysis
Ⅲ. RESULTS
    1. Hypoxia induces changes in EMT-relatedmRNA expression profiles of OSCC cells
    2. Hypoxia downregulated transcription andtranslation of TCF-4 in OSCC cells
    3. Hypoxia suppressed β-catenin signalingdespite the upregulation of β-catenin underhypoxic conditions in OSCC cells
Ⅳ. DISCUSSION
REFERENCES
저자
  • 박대근(부산대학교 치의학전문대학원 구강병리학교실/BK21 Plus/치주질환신호네트워크연구센터) | Dae-Gun Park (Department of Oral Pathology, BK21 Plus Project, Periodontal Disease Signaling Network Research Center (MRC), School of Dentistry, Pusan National University)
  • 김소라(부산대학교 치의학전문대학원 구강병리학교실/BK21 Plus/치주질환신호네트워크연구센터) | So Ra Kim (Department of Oral Pathology, BK21 Plus Project, Periodontal Disease Signaling Network Research Center (MRC), School of Dentistry, Pusan National Universit)
  • 우복희(부산대학교 치의학전문대학원 구강병리학교실/BK21 Plus/치주질환신호네트워크연구센터) | Bok Hee Woo (Department of Oral Pathology, BK21 Plus Project, Periodontal Disease Signaling Network Research Center (MRC), School of Dentistry, Pusan National University)
  • 이지혜(부산대학교 치의학전문대학원 구강병리학교실/BK21 Plus/치주질환신호네트워크연구센터) | Ji Hye Lee (Department of Oral Pathology, BK21 Plus Project, Periodontal Disease Signaling Network Research Center (MRC), School of Dentistry, Pusan National University)
  • 박혜련(부산대학교 치의학전문대학원 구강병리학교실/BK21 Plus/치주질환신호네트워크연구센터) | Hae Ryoun Park (Department of Oral Pathology, BK21 Plus Project, Periodontal Disease Signaling Network Research Center (MRC), School of Dentistry, Pusan National University) Correspondence