KOREASCHOLAR

두경부 암종세포에서 Luzindole을 이용한 YD15 세포와 HSC5 세포의 상피간엽이행 발현 변화 분석 The Effects of Luzindole on Cells Migration and Invasion and Epithelial-mesenchymal Transition in YD15 and HSC5 Head and Neck Cancer Cells

장민아, 김욱규, 정승화, 한혜연, 유미현
  • 언어ENG
  • URLhttp://db.koreascholar.com/Article/Detail/405767
대한구강악안면병리학회지
제45권 제1호 (2021.02)
pp.11-20
대한구강악안면병리학회 (Korean Academy Of Oral And Maxillofacial Pathology)
초록

Human melatonin receptors consist of melatonin receptor 1A (MT1) and melatonin receptor 1B (MT2), and possess various biological activations, which include the control of circadian rhythm and immune regulation. Recently, it have been found that melatonin receptors inhibit cell proliferation and have oncostatic properties, which is being researched in the treatment strategies of breast cancer, prostate cancer, and Non-Small Cell Lung Cancer. Also, interest in the effect of melatonin receptor’s correlation to head and neck carcinogenesis and application possibilities on head and neck cancer has been found. However, in head and neck cancer, how melatonin receptor relates and functions with epithelial-mesenchymal transition (EMT), which plays a major role in human carcinogenesis, is yet unknown. In this research, in HSC5 cell and YD15 cell, the head and neck cancer cell lines, a selective melatonin receptor antagonist, Luzindole, was utilized to examine the effect of melatonin receptors on EMT. After treating Luzindole on HSC5 cells and YD15 cells, the authors evaluated cell viability rate with CCK 8 assay, and performing colony forming assay, invasion assay and western blot analysis, to confirm melatonin receptor’s effect on EMT. When Luzindole was treated on HSC5 cells and YD15 cells in low concentration of 100nM, no significant difference in cell viability was found, whereas Luzindole-treated cells had a significantly increase in the invasion assay. As a result of colony forming assay, in YD15 cells, the number of colony formation decreased slightly, whereas in HSC3 cells, the number of colony formation increased. According to the western blotting, no difference in E-cadherin, Slug, and vimentin protein expression was shown. This result of research indicates the possibility of melatonin receptor being related to EMT and new chemotherapeutic target in the carcinogenesis of head and neck cancer.

목차

Ⅰ. INTRODUCTION
Ⅱ. SUBJECTS AND METHODS
    1. Reagents and antibodies
    2. Cell culture
    3. Luzindole treatment
    4. CCK-8 assay
    5. Colony forming assay
    6. Invasion assay
    7. Western blot analysis
    8. Statistical Analysis
Ⅲ. RESULTS
    1. CCK-8 assay in HSC5 and YD15 cells
    2. Luzindole treatment inhibits invasivecapacity in HSC5 and YD15 cell lines
    3. Luzindole treatment is involved in colonyforming potential in HSC5 and YD15 celllines
    4. Luzindole treatment did not involved in theexpression of E-cadherin, Slug and vimentinproteins in HSC5 and YD15 cell lines
Ⅳ. DISCUSSION
Ⅴ. CONCLUSION
REFERENCES
저자
  • 장민아(부산대학교 치의학전문대학원 구강병리학 교실, 치의생명과학연구소) | MinA Jang (Department of Oral Pathology, School of Dentistry, Dental and Life Science Institute, Pusan National University)
  • 김욱규(부산대학교 치의학전문대학원 구강악안면외과학 교실) | Uk-Kyu Kim (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University)
  • 정승화(부산대학교 치의학전문대학원 예방과 사회치의학 교실) | Seung-hwa Jeong (Department of Preventive & Community Dentistry, School of Dentistry, Pusan National University)
  • 한혜연(진주보건대학교 치위생과) | Hye-Yeon Han (Department of Dental Hygeine, Jinju Health Collge)
  • 유미현(부산대학교 치의학전문대학원 구강병리학 교실, 부산대학교 치의생명과학 교육연구팀, 치의생명과학연구소) | Mi Heon Ryu (Department of Oral Pathology, Education and Research Team for Life Science on Dentistry, School of Dentistry, Dental and Life Science Institute, Pusan National University) Correspondence