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Chlorhexidine digluconate가 세균외막과 인공세포막의 회전운동에 미치는 비대칭적 영향 Amphiphilic Effects of Chlorhexidine Digluconate on Rotational Mobility in Bacterial Outer Membranes and Liposomes

김태영, 차성권, 엄승일, 배문경, 배수경, 주지민, 윤일희, 김다솔, 장혜옥
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  • URLhttp://db.koreascholar.com/Article/Detail/408799
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대한구강악안면병리학회지
제45권 제3호 (2021.06)
pp.77-85
대한구강악안면병리학회 (Korean Academy Of Oral And Maxillofacial Pathology)
초록

Chlorhexidine is a cationic bisbiguanide with broad antibacterial activity, and diverse activity encompassing gram-positive and gram-negative bacteria, yeasts, dermatophytes, and some lipophilic viruses. Chlorhexidine alters the permeability of the bacterial membrane. They also neutralize periodontal pathogens such as Streptococcus aureus, Porphyromonas gingivalis, and Prevotella intermedia. Porphyromonas gingivalis were cultured to isolate the Porphyromonas gingivalis outer membranes (OPG). Also, OPG were used to prepare large unilamellar liposomes with total lipids (OPGTL) extracted and prepared with the phospholipids mixture (PL). The effect of chlorhexidine digluconate on cell membrane kinetic changes was investigated using fluorescence polarization of n-(9-anthroyloxy) stearic acid with different rotational mobility depending on the probe substitution position (n) in the membrane phospholipid aliphatic chain. Chlorhexidine digluconate increased the hydrocarbon rotational mobility interior of the OPG, OPGTL and PL, but native and model membranes interfacial mobility were decreased. The sensitivity to increasing chlorhexidine digluconate effect on rotational mobility was proportional to the depth of probe position in descending order of 16-AP, 12-AS, 6-AS and 9-AS. Chlorhexidine digluconate disordering or ordering the effects on the membrane lipids may cause its bacteriostatic and bacteriocidal actions.

키워드
Bacterial outer membranes and liposomesChlorhexidine digluconateFluorescence probe techniqueHydrocarbon interiorMembrane interface
목차

Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
    1. Matarials
    2. Bacterial growth conditions
    3. Isolation of outer membrane
    4. Extraction of lipids from OPG
    5. Liposome preparation
Ⅲ. RESULTS
    1. Disordering effects of chlorhexidinedigluconate on the rotational mobility of thehydrocarbon interior
    2. Ordering effects of chlorhexidine digluconateon the rotational mobility of the hydrocarboninterior
Ⅳ. DISCUSSION
REFERENCES
저자
  • 김태영(부산대학교 치의학전문대학원 치과약리학교실) | Tae Young Kim (Department of Dental Pharmacology, Pusan National University School of Dentistry)
  • 차성권(부산대학교 치의학전문대학원 치과약리학교실) | Seong-Kweon Cha (Department of Dental Pharmacology, Pusan National University School of Dentistry)
  • 엄승일(부산대학교 치의학전문대학원 치과약리학교실) | Seung-Il Eom (Department of Dental Pharmacology, Pusan National University School of Dentistry)
  • 배문경(부산대학교 치의학전문대학원 구강생리학교실/부산대학교 치의학전문대학원 치주질환네크워크신호전달센터 (MRC)) | Moon-Kyoung Bae (Department of Oral Physiology, Pusan National University School of Dentistry/Periodontal Disease Signaling Network Research Center (MRC), School of Dentistry, Pusan National University)
  • 배수경(부산대학교 치의학전문대학원 치과약리학교실/부산대학교 치의학전문대학원 치주질환네크워크신호전달센터 (MRC)) | Soo-Kyung Bae (Department of Dental Pharmacology, Pusan National University School of Dentistry/Periodontal Disease Signaling Network Research Center (MRC), School of Dentistry, Pusan National University)
  • 주지민(부산대학교 치의학전문대학원 치과약리학교실) | Ji-min Ju (Department of Dental Pharmacology, Pusan National University School of Dentistry)
  • 윤일희(부산대학교 치의학전문대학원 치과약리학교실) | Il Yun (Department of Dental Pharmacology, Pusan National University School of Dentistry)
  • 김다솔(부산대학교 치의학전문대학원 치과약리학교실/부산대학교 치의학전문대학원 치주질환네크워크신호전달센터 (MRC)) | Da-Sol Kim (Department of Dental Pharmacology, Pusan National University School of Dentistry/Periodontal Disease Signaling Network Research Center (MRC), School of Dentistry, Pusan National University)
  • 장혜옥(부산대학교 치의학전문대학원 치과약리학교실) | Hye-Ock Jang (Department of Dental Pharmacology, Pusan National University School of Dentistry) Correspondence