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Correlations between Sperm Motility, SCSA (Sperm Chromatin Structure Assay) , Reproductive Performance and Heterospermic Fertility in Boars

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  • URLhttps://db.koreascholar.com/Article/Detail/109714
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한국동물번식학회 (The Korean Society of Animal Reproduction)
초록

The objective of this study was two folds: to investigate the relationship between paternal identification rate and sperm quality parameters such as motility and sperm chromatin structure assay after heterospermic insemination; to see if mutual complement between tests and development of useful technique to enhance the fertility in artificial insemination. In individual boar's fertilizing ability, 3 high fertility boars showed significantly high fertility (p<0.05) compared to 3 low fertility boars, but there was no difference in litter size between two groups. Sperm motility test in pooled and individual semen using computer assisted sperm analysis (CASA) revealed that no significant difference among boars. The high fertile boar showed tendency of low %Red (High red fluorescence/green+red fluorescence) in sperm chromatin structure assay (SCSA) but paternal identification rate from piglets did not differ after heterospermic insemination. The correlation coefficient between individual or pooled semen function test and farrowing rates were well correlated as follows: %Red with litter size (r= - 0.53, p=0.03); %Red with paternal identification rates (r=-0.51, p=0.03); paternal identification rates with litter size (r=0.57, p=0.02). These results indicate that sperm chromatin structure assay and sperm quality parameter test in pooled semen are useful method to predict and evaluate the fertilizing capacity after heterospermic insemination in boars.

목차
ABSTRACT   IBTRODUCTION   MATERIALS AND METHODS   RESULTS   DISCUSSION   REFERENCES
저자
  • In Cheul Kim(National Institute of Animal Science, RDA)
  • Jae Weon Ryu(National Institute of Animal Science, RDA) Corresponding author
  • Kyu Ho Cho(National Institute of Animal Science, RDA)
  • Joon-Ki Hong(National Institute of Animal Science, RDA)
  • Eun Ji Choi(National Institute of Animal Science, RDA)
  • Bong-Hwan Choi(National Institute of Animal Science, RDA)
  • Jun Cheol Park(National Institute of Animal Science, RDA)
  • Hong-Kil Moon(National Institute of Animal Science, RDA) | Hong-KIl Moon
  • Jung-Ho Son(Noah Biotech Inc)