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비글(Beagle)종 개 정액의 성상 및 보존성
The Semen Propery and Preservation in Beagle Dogs
This study was carried out to investigate the general characteristics, such as volume, pH, sperm motility and sperm concentration of the semen collected from Beagle dogs (age 24～48 months, weight 10～15 kg) by using the method of digital manipulation of the penis, and the effect of preservation temperature and time on motility of fresh semen. Multiple ejaculates were collected from 4 male Beagles. The average volume, pH, motility and sperm concentration of the second fraction (contained with small volume of the third fraction) per ejaculation were 2.94±0.24(SD) ml, 6.43±0.42(SD), 97.04±3.50(SD)% and 1.67±0.23(SD)×108 cells/ml, respectively. Average semen volume per ejaculate, semen pH, sperm motility and sperm concentration of the first fraction from the ejaculate were 1.24±0.20(SD) ml, 6.03±0.26(SD), 11.30±4.02(SD)% and 7.25±1.02(SD)×105 cells/ml. Those of second fraction were 2.52± 0.32(SD) ml, 6.32±0.31(SD), 96.25±3.52(SD)% and 2.35±0.35(SD)×108 cells/ml. Those of third fraction were 2.71±0.27 (SD) ml, 6.52±0.20(SD), 95.65±2.78(SD)% and 5.72±0.29(SD)×107 cells/ml. Motility of semen was higher at 17℃ preservation temperature than 5℃ or 36℃ during preservation period. When preservation temperature was 17℃, motility was 96.54±2.05(SD)% at 1 h, 90.20±3.90(SD)% at 6 h, 89.05±2.01(SD)% at 12 h, 78.21±3.50(SD)% at 18 h, 45.24±6.25 (SD)% at 24 h and 30.75±17.24(SD)% at 30 h, respectively.
