Oocyte enucleation is essential for somatic cell nuclear transfer (SCNT) in the production of cloned animals or embryonic stem cells from adult somatic cells. Most studies of oocyte enucleation have been performed using micromanipulator-based techniques, which are technically demanding, time-consuming, and expensive. Several recent studies have used chemical-induced oocyte enucleation; however, each has been plagued by low efficiency and toxicity. In this study, I found that the co-treatment of murine oocytes with demecolcine and BMI-1026, a potent cdk1 inhibitor, resulted in a high enucleation rate (97%). This method is entirely independent of a micromanipulator and is suitable for the large-scale production of enucleated oocytes. This new method of enucleation will be useful in SCNT and in the development of handmade cloning techniques.

ABSTRACT   INTRODUCTION   MATERIALS AND METHODS    Collection of Ovulated Oocytes    Induction of Oocyte Enucleation and Evaluation    Oocyte Fixation and DNA Staining    Classification of the Phenotypes of the Oocytes    Statistical Analysis   RESULTS    Classification of the Oocytes into Four Types   DISCUSSION   REFERENCES

• 최태생(단국대학교) | Taesaeng Choi Corresponding author