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BTS와 Androhep이 보존 기간 동안 액상 정액의 운동역학 및 수정능 획득에 미치는 영향

Effect of BTS and Androhep during Storage Times on the Kinematics and Capacitation Status in Liquid Boar Semen

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  • URLhttps://db.koreascholar.com/Article/Detail/163420
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한국동물번식학회 (The Korean Society of Animal Reproduction)
초록

The objective of this study was to investigate the effect of storage time on fresh boar semen in Androhep and Beltsville Thawing Solution (BTS). Boar semen samples extended in each extender were stored at 17℃ up to 4 days. Sperm motility kinematics was evaluated by computer assisted sperm analyzer (CASA) and capacitation status by chlortetracycline (CTC)/Hoechst 33258 staining. Sperm motility (%) was not decreased during storage in BTS and Androhep. No significant difference between extenders was observed. Only significant differences in kinematic parameters on linearity during storage were found. The percentage of dead sperm significantly decreased during storage (p<0.05). Also the percentage of noncapacitated, capacitated, and acrosome-reacted sperm significantly modified during storage (p<0.05). However, there was no significant difference between extenders except proportion of capacitated sperm. This finding supported that modification in these parameters was not significantly different between extenders during this short-term storage. Our finding strongly indicated that both Androhep and BTS maintained favorable conditions for motility, motility kinematics, and capacitation status during short-term storage. Despite modifications in some parameters were apparent during sperm storage in extenders, these may not affect the fertilizing capacity of boar semen.

목차
ABSTRACT   서론   재료 및 방법    액상 정액    CASA를 이용한 운동성 측정    CTC/H33258 Staining을 이용한 침체막의 변화 측정    통계 분석   결과    보존기간 동안 회석액에 따른 정자의 운동성 변화    보존 기간 동안 회석액에 따른 정자의 수정능 획득과 침제막의 변화   고찰   인용문헌
저자
  • 김연희(중앙대학교 산업과학대학 동물자원과학과) | Yun-Hee Kim
  • 박유진(중앙대학교 산업과학대학 동물자원과학과) | Yoo-Jin Park
  • 윤성재(중앙대학교 산업과학대학 동물자원과학과) | Sung-jae Yoon
  • 권우성(중앙대학교 산업과학대학 동물자원과학과) | Woo-Sun Kwon
  • 김상현((주)다비AI센터) | Sang Hyun Kim
  • 방명걸(중앙대학교 산업과학대학 동물자원과학과, 중앙대학교 생명환경연구원) | Myung-Geol Pang Corresponding author