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Developmental Expression of Plasma Glutathione Peroxidase during Mouse Organogenesis

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/191582
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발생공학 국제심포지엄 및 학술대회 (International Symposium on Developmental Biotechnology)
한국동물번식학회 (The Korean Society of Animal Reproduction)
초록

Plasma glutathione peroxidase (pGPx) is an extracellular antioxidative selenoenzyme which has been detected in various adult tissues, but little is known about the expression and distribution of pGPx during embryogenesis. To investigate the expression patterns of pGPx during embryogenesis, we performed quantitative real-time PCR, in situ hybridization, Western blot, and immunohistochemistry analyses in whole embryos or each developing organ of mice on embryonic days (E)7.5–18.5. In whole embryos of E7.5–8.5, pGPx mRNA was more typically expressed in extra-embryonic tissues including ectoplacental cone, trophectoderm, and decidual cells than in embryos. However, after E9.5, pGPx mRNA and protein levels were increased in the embryos with differentiation and growth, but trended to gradually decrease in the extra-embryonic tissues until E18.5. In sectioned embryonic tissues on E13.5–18.5, pGPx mRNA and protein were mainly expressed in the developing nervous tissues, the sensory organs, and the epithelia of lung, skin, and intestine, the heart and artery, and the kidney. In particular, pGPx immunoreactivity was very strong in the developing liver. These results indicate that pGPx is spatio-temporally expressed in various embryonic organs as well as extra-embryonic tissues, suggesting that pGPx may function to protect the embryos against endogenous and exogenous reactive oxygen species during organogenesis.

저자
  • Ki Youn Jung(College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • In-Jeoung Baek(Laboratory of Mammalian Molecular Genetics, Department of Biochemistry, College of Science, Yonsei University)
  • Jung-Min Yon(College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • Beom Jun Lee(College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • Young Won Yun(College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • Sang-Yoon Nam(College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)