The experimental manipulation of protooncogenes and their gene products is a valuable research tool for the study of human neoplasia. In this study, the recently identified human cervical cancer protooncogene (HccR-2) was expressed in transgenic mice under the control of the tetracycline regulatory system. Mice expressing the HccR-2 transgene showed an altered myeloid development characterized by an increased percentage of mature and band-form neutrophils in the peripheral blood, liver and spleen. This phenotype is similar to human chronic neutrophilic leukemia (CNL) in many ways, which is a rare chronic myeloproliferative disorder (CMD) that presents as a sustained leukocytosis of mature neutrophils with a few or no circulating immature granulocytes, an absence of peripheral blood monocytosis, basophilia, or eosinophilia, and an infiltration of neutrophils into the liver, spleen and kidney. Thus, the HccR-2 transgenic mouse model is imperative not only for investigating the biological properties of the HccR-2 protooncogene in vivo, but also for analyzing the mechanisms involved in the progression of CNL.

ABSTRACT   INTRODUCTION   MATERIALS AND METHODS    Generation of Transgenic Mice    PCR Analysis    Southern Blot Hybridization Analysis    RNA Analysis by Reverse Transcriptase-PCR (RTPCR)    Western Blot Hybridization Analysis    Morphologic and Histologic Analyses    Statistical Analysis   RESULTS    Production of the Transgenic Mice Expressing Hcc-R-2    Clinical and Laboratory Diagnosis    Development of Chronic Neutrophilic Leukemia(CNL)   DISCUSSION   REFERENCES

• Byoung Boo Seo(Dept. of Animal Resources, College of Life & Environmental Science, Daegu University)
• Humdai Park(Dept. of Biotechnology, College of Engineering, Daegu University) Corresponding author