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KCI 등재
Ex Vivo Assay of Trace Nicotine Using a Voltammetric Modified Biosensor
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/257906
pp.40-46
In vivo nicotine is associated with Alzheimer's, Parkinson's and lung cancer. Diagnostic assays of these diseases depend on very low analytical detection limits. In this study, a sensitive analytical method was examined using a voltammetric graphite pencil electrode (GPE) and a modified carbon nanotube paste electrode (CNE). The optimum analytical conditions for both electrodes were compared using square wave anodic stripping voltammetry (SW) and cyclic voltammetry (CV) obtaining 400 sec accumulation time and oxidation peak. Under optimum parameters, the stripping working range of GPE was 5.0-40.0μg/L, CNE: 0.1-0.8 and 5-50μg/L. Quantification limits were 5.0μg/L for GPE and 0.1μg/L for CNE, while detection limits were 0.6μg/L for GPE and 0.07μg/L for CNE. A standard deviation of 10.0μg/L was observed for 0.064 GPE and 0.095 CNE (n = 12) using 400 sec accumulation time. The results obtained can be applied to non.treated urine and ex vivo biological diagnostics.
