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Tetrapolar characteristics of mating types in Pleurotus ostreatus strain
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/277136
A Pleurotus ostreatus strain ‘CM0109068’ showing gray and semispherical in color and shape of pileus were examined in its tetrapolarity of mating types. Single basidiospores were isolated from its fruiting body and monokaryotic mycelia were obtained from each isolate. Mating experiments were performed using 22 monokaryons to provide mating compatibility data. Pairings were performed in the petri dishes on PDA and they were allowed to grow at 25℃ until two fronts of the advancing mycelia met and developed a conspicuous contact zone. The contact zone and the outer edges of paired colonies on each plate were examined for clamp connections and the crosses were considered dikaryotized when clamp connections were found at the growing margin of either side of the interacting monokaryons. The tested monokaryons resulted in tetrapolar incompatibility in intrastrain crosses. Compatible matings were distinguished from incompatible ones by the rapid growth and gross morphology. The dikaryotic mycelium colony was also confirmed microscopically by the presence of clamp connections from incompatible pairings. The four classes of tester strains representing the four incompatibility types were chosen from intrastrain mating tests. The mating tests with the tester strain were performed for the determination of the rest monokaryons and as a result of these tests it was found that the strain share the same A and B incompatibility factors showing tetrapolar incompatibility characteristics.
