논문 상세보기

Cathepsin B Inhibitor, E-64, Affects Preimplantation Development, Apoptosis and Oxidative Stress in Pig Embryos

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/280004
구독 기관 인증 시 무료 이용이 가능합니다. 4,000원
한국동물번식학회 (The Korean Society of Animal Reproduction)
초록

Cathepsin B is abundantly expressed peptidase of the papain family in the lysosomes, and closely related to the cell degradation system such as apoptosis, necrosis and autophagy. Abnormal degradation of organelles often occurs due to release of cathepsin B into the cytoplasm. Many studies have been reported that relationship between cathepsin B and intracellular mechanisms in various cell types, but porcine embryos has not yet been reported. Therefore, this study evaluated the effect of cathepsin B inhibitor (E-64) on preimplantation developmental competence and quality of porcine embryos focusing on apoptosis and oxidative stress. The expression of cathepsin B mRNA in porcine em-bryos was gradually decreased in inverse proportion to E-64 concentration by using real-time RT-PCR. When putative zygotes were cultured with E-64 for 24 h, the rates of early cleavage and blastocyst development were decreased by increasing E-64 concentration. However, the rate of blastocyst development in 5 μM treated group was similar to the control. On the other hand, both the index of apoptotic and reactive oxygen species (ROS) of blastocysts were sig-nificantly decreased in the 5 μM E-64 treated group compared with control. We also examined the mRNA expression levels of apoptosis related genes in the blastocysts derived from 5 μM E-64 treated and non-treated groups. Expre-ssion of the pro-apoptotic Bax gene was shown to be decreased in the E-64 treated blastocyst group, whereas expre-ssion of the anti-apoptotic Bcl-xL gene was increased. Taken together, these results suggest that proper inhibition of cathepsin B at early development stage embryos improves the quality of blastocysts, which may be related to not only the apoptosis reduction but also the oxidative stress reduction in porcine embryos.

저자
  • Hyeong-Hoon Son(Department of Biotechnology, Daegu University)
  • Sung-Hun Min(Department of Biotechnology, Daegu University)
  • Ji-Yeong Yeon(Department of Biotechnology, Daegu University)
  • Jin-Woo Kim(Department of Biotechnology, Daegu University)
  • Soo-Yong Park(Department of Biotechnology, Daegu University)
  • Yong-Hee Lee(Department of Biotechnology, Daegu University)
  • Pil-Soo Jeong(Department of Biotechnology, Daegu University)
  • Deog-Bon Koo(Department of Biotechnology, Daegu University) Corresponding author